Genome-wide characterization and expression profiling of MAPK cascade genes in Salvia miltiorrhiza reveals the function of SmMAPK3 and SmMAPK1 in secondary metabolism
Genome-wide characterization and expression profiling of MAPK cascade genes in Salvia miltiorrhiza reveals the function of SmMAPK3 and SmMAPK1 in secondary metabolism
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DOI：
10.1186/s12864-020-07023-w
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发表时间：
2020-09
期刊：
影响因子：
4.4
通讯作者：
Yongfeng Xie;Mei-mei Ding;Bin Zhang;Jie Yang;Tianlin Pei;Pengda Ma;Juane Dong
中科院分区：
文献类型：
--
作者：
Yongfeng Xie;Mei-mei Ding;Bin Zhang;Jie Yang;Tianlin Pei;Pengda Ma;Juane Dong
背景
Background
The contribution of mitogen-activated protein kinase (MAPK) cascades to plant growth and development has been widely studied, but this knowledge has not yet been extended to the medicinal plant Salvia miltiorrhiza, which produces a number of pharmacologically active secondary metabolites.
Results
In this study, we performed a genome-wide survey and identified six MAPKKK kinases (MAPKKKKs), 83 MAPKK kinases (MAPKKKs), nine MAPK kinases (MAPKKs) and 18 MAPKs in the S. miltiorrhiza genome. Within each class of genes, a small number of subfamilies were recognized. A transcriptional analysis revealed differences in the genes’ behaviour with respect to both their site of transcription and their inducibility by elicitors and phytohormones. Two genes were identified as strong candidates for playing roles in phytohormone signalling. A gene-to-metabolite network was constructed based on correlation analysis, highlighting the likely involvement of two of the cascades in the synthesis of two key groups of pharmacologically active secondary metabolites: phenolic acids and tanshinones.
Conclusion
The data provide insight into the functional diversification and conservation of MAPK cascades in S. miltiorrhiza.