EVALUATION OF 31 MOUSE MONOCLONAL-ANTIBODIES TO HUMAN-IGG EPITOPES
EVALUATION OF 31 MOUSE MONOCLONAL-ANTIBODIES TO HUMAN-IGG EPITOPES
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DOI：
10.1089/hyb.1984.3.263
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
MCDOUGAL, JS
中科院分区：
文献类型：
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作者：
REIMER, CB;PHILLIPS, DJ;MCDOUGAL, JS
获得了31个产生抗人IgG抗原决定簇的单克隆抗体（MAb）的小鼠杂交瘤的稳定克隆。所描述的不同特异性的杂交瘤的数目是：2个抗-IgG 1 Fc，1个抗-IgG 2 Fc，1个抗-IgG 2 Fd，2个抗-IgG 3 Fc，2个抗-IgG 3铰链，3个抗-IgG 4 Fc，3个抗-IgG 4 Fd，2个抗-nG 4 m（B），4个抗-IgGFc，2个抗-IgGFd，抗-κ，1个抗-λ，1个抗-非IgG 1，2个抗-非IgG 2，2个抗-非IgG 3，2抗非IgG 4。有证据证明其特异性。一些被证明对明确定义的IgG亚类表位具有亲和力、效力和特异性的单克隆抗体在其他检测系统中可能部分或完全无活性，可能是因为抗原表位的不同呈现。一般来说，这个问题需要仔细编写描述单克隆抗体使用的方案。
Stable clones of 31 mouse hybridomas that produce monoclonal antibodies (MAb) against human IgG antigenic determinants were obtained. The number of hybridomas of different specificity described are: 2 anti-IgG1 Fc, 1 anti-IgG2 Fc, 1 anti-IgG2 Fd, 2 anti-IgG3 Fc, 2 anti-IgG3 hinge, 3 anti-IgG4 Fc, 3 anti-IgG4 Fd, 2 anti-nG4m(b), 4 anti-IgGFc, 2 anti-IgGFd, anti-kappa, 1 anti-lambda, 1 anti-non IgG1, 2 anti-non IgG2, 2 anti-non-IgG3, 2 anti-non-IgG4. Evidence is presented validating their specificity. Some MAb demonstrated to be avid, potent and specific for well defined IgG-subclass epitopes may be partially or completely inactive in other assay systems, presumably because of different presentations of antigen epitopes. In general, this problem requires careful writing of protocols describing the use of MAb.