Sex-biased transcriptomic response of the reproductive axis to stress
Sex-biased transcriptomic response of the reproductive axis to stress
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DOI：
10.1016/j.yhbeh.2017.11.011
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发表时间：
2018-04-01
影响因子：
3.5
通讯作者：
MacManes, Matthew D.
中科院分区：
文献类型：
--
作者：
Calisi, Rebecca M.;Austin, Suzanne H.;MacManes, Matthew D.
众所周知，压力是许多物种（包括鸟类、啮齿动物和人类）生殖功能障碍的原因，尽管雄性和雌性的反应可能不同。研究压力如何影响生殖的一个有效方法是检查它对调节生殖所必需的生物系统的影响，即下丘脑-垂体-性腺（HPG）轴。通常，这是通过观察压力如何影响血液中循环的糖皮质激素（如皮质醇或皮质酮）的数量以及它们与少数已知的产生HPG的生殖激素（如睾酮和雌二醇）的关系来实现的。到目前为止，我们对压力如何影响HPG轴的所有基因组活动以及这在性别之间可能存在何种差异缺乏充分的了解。我们利用一种高度重复和性别平衡的实验方法来测试雄性和雌性岩鸽（Columba livia）如何在转录组水平上对约束压力做出反应。与男性相比，女性在其HPG轴的所有水平上表现出对压力的基因组响应性增加，并且这些响应基因大多是女性独有的。其原因可能是由于女性内分泌环境在生殖周期和/或其进化史中的波动，包括父母的投资和对母亲的影响。在这个阶段，基因组与表型组因果关系之间的直接联系无法确定;然而，我们报告的数据提供了一个重要的基因组基础，在此基础上，可以进一步实验研究性别特异性生殖功能障碍和面对压力的适应，以及用于遗传干预和治疗研究的新基因靶点。
Stress is a well-known cause of reproductive dysfunction in many species, including birds, rodents, and humans, though males and females may respond differently. A powerful way to investigate how stress affects reproduction is by examining its effects on a biological system essential for regulating reproduction, the hypothalamic-pituitary-gonadal (HPG) axis. Often this is done by observing how a stressor affects the amount of glucocorticoids, such as cortisol or corticosterone, circulating in the blood and their relationship with a handful of known HPG-producing reproductive hormones, like testosterone and estradiol. Until now, we have lacked a full understanding of how stress affects all genomic activity of the HPG axis and how this might differ between the sexes. We leveraged a highly replicated and sex-balanced experimental approach to test how male and female rock doves (Columba livia) respond to restraint stress at the level of their transcriptome. Females exhibit increased genomic responsiveness to stress at all levels of their HPG axis as compared to males, and these responsive genes are mostly unique to females. Reasons for this may be due to fluctuations in the female endocrine environment over the reproductive cycle and/or their evolutionary history, including parental investment and the potential for maternal effects. Direct links between genome to phenome cause and effect cannot be ascertained at this stage; however, the data we report provide a vital genomic foundation on which sex-specific reproductive dysfunction and adaptation in the face of stress can be further experimentally studied, as well as novel gene targets for genetic intervention and therapy investigations.
分泌组织的肌球蛋白。
影响因子：
7.8
作者：
Ostlund, R E Jr;Leung, J T;Kipnis, D M
通讯作者：
Kipnis, D M