Sodium hyaluronate-functionalized urea-formaldehyde monolithic column for hydrophilic in-tube solid-phase microextraction of melamine
Sodium hyaluronate-functionalized urea-formaldehyde monolithic column for hydrophilic in-tube solid-phase microextraction of melamine
复制标题
透明质酸钠功能化脲醛整体柱亲水管内固相微萃取三聚氰胺
DOI：
10.1016/j.chroma.2017.08.005
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发表时间：
2017
影响因子：
4.1
通讯作者：
Qixing Zhang
中科院分区：
文献类型：
--
作者：
Wang Jiabin;Jiang Nan;Cai Zhengmiao;Li Wenbang;Li Jianhua;Lin Xucong;Xie Zenghong;Qixing Zhang
采用尿素、甲醛和透明质酸钠原位缩聚的方法，制备了一种新型的透明质酸钠功能化脲醛整体柱。透明质酸具有交联和亲水性功能化的双重作用。通过对不同分子量的透明质酸和不同用量的透明质酸的制备因素进行优化，得到了具有良好渗透性和亲水性的均匀单体。由于HA具有优异的亲水性，HA功能化UF单体比UF单体具有更高的亲水性萃取效率，并应用于三聚氰胺（MEL）的管内亲水固相微萃取（SPME）。考察了进样液中ACN的百分比、进样液的盐浓度和pH值、洗脱体积、取样和洗脱流速等因素对MEL萃取效率的影响。在优化条件下，MEL的检出限为0.2 ng/mL，加标回收率为87.3% ~ 96.7%，相对标准偏差（rsd）小于5.1%。由于具有良好的亲水性，新型ha功能化UF单体可以为复杂样品中极性分析物的灵敏分析提供一种很有前景的工具。
A novel sodium hyaluronate-functionalized urea-formaldehyde (UF) monolithic column has been developed by in-situ polycondensation of urea, formaldehyde and sodium hyaluronate (HA). HA plays both the roles of crosslinking and hydrophilic functionalization. The preparation factors including different molecular weights of HA and different amounts of HA were optimized, and then a uniform monolith with satisfactory permeability and hydrophilic binding capacity was obtained. Due to the excellent hydrophilicity of HA, HA-functionalized UF monolith showed higher hydrophilic extraction efficiency than UF monolith, and was applied for hydrophilic in-tube solid-phase microextraction (SPME) of melamine (MEL). Several factors for hydrophilic in-tube SPME, such as ACN percentage in the sampling solution, salt concentration and pH value of the sampling solution, elution volume, sampling and elution flow rate, were investigated with respect to the extraction efficiency of MEL. Under the optimized SPME conditions, the limit of detection (LOD) of MEL was found to be 0.2 ng/mL in the milk formula samples, the recoveries of MEL spiked in milk formula samples ranged from 87.3% to 96.7% with relative standard deviations (RSDs) less than 5.1%. Owing to the excellent hydrophilic extraction ability, the novel HA-functionalized UF monolith could provide a promising tool for the sensitive analysis of polar analytes in complicated samples.