Phylogenetic comparison of 5′ splice site determination in central spliceosomal proteins of the U1-70K gene family, in response to developmental cues and stress conditions
Phylogenetic comparison of 5′ splice site determination in central spliceosomal proteins of the U1-70K gene family, in response to developmental cues and stress conditions
复制标题
DOI：
10.1111/tpj.14735
复制
发表时间：
2020-04-24
期刊：
影响因子：
7.2
通讯作者：
Fang, Yan-Ming
中科院分区：
文献类型：
--
作者：
Chen, Mo-Xian;Zhang, Kai-Lu;Fang, Yan-Ming
含有内含子的基因具有通过剪接产生多种转录异构体的能力，从而极大地扩展真核生物的转录组和蛋白质组。在真核细胞中，前体mRNA（pre-mRNA）剪接是由被定义为剪接体的大分子复合物进行的。U1小核核糖核蛋白（U1 small nuclear ribonucleoprotein，U1 snRNP）是参与剪接体早期组装和5 '-剪接位点识别的最小的亚复合物。它的核心成分，命名为U1 - 70K，已在动物和酵母中广泛表征。然而，对U1 - 70K基因在植物中的研究很少。为此，我们进行了全面的研究，系统地确定了115 U1 - 70K基因从67种植物，从藻类到被子植物。系统发育分析表明，植物U1 - 70K基因家族的扩展可能是由全基因组复制驱动的。随后的基因结构，蛋白质结构域，启动子区域和保守的剪接模式的比较表明，植物U1 - 70 Ks很可能保持其保守的分子功能在植物谱系和响应环境胁迫中发挥重要的功能作用。此外，利用T-DNA插入突变体进行的遗传分析表明，拟南芥U1 - 70K可能参与了对渗透胁迫的响应。我们的研究结果提供了一个总体概述，这个基因家族在Viridiplantae，并将作为一个参考来源，为未来的机制研究，这个U1 snRNP特异性剪接因子。
Intron-containing genes have the ability to generate multiple transcript isoforms by splicing, thereby greatly expanding the eukaryotic transcriptome and proteome. In eukaryotic cells, precursor mRNA (pre-mRNA) splicing is performed by a mega-macromolecular complex defined as a spliceosome. Among its splicing components, U1 small nuclear ribonucleoprotein (U1 snRNP) is the smallest subcomplex involved in early spliceosome assembly and 5 '-splice site recognition. Its central component, named U1-70K, has been extensively characterized in animals and yeast. Very few investigations on U1-70K genes have been conducted in plants, however. To this end, we performed a comprehensive study to systematically identify 115 U1-70K genes from 67 plant species, ranging from algae to angiosperms. Phylogenetic analysis suggested that the expansion of the plant U1-70K gene family was likely to have been driven by whole-genome duplications. Subsequent comparisons of gene structures, protein domains, promoter regions and conserved splicing patterns indicated that plant U1-70Ks are likely to preserve their conserved molecular function across plant lineages and play an important functional role in response to environmental stresses. Furthermore, genetic analysis using T-DNA insertion mutants suggested that Arabidopsis U1-70K may be involved in response to osmotic stress. Our results provide a general overview of this gene family in Viridiplantae and will act as a reference source for future mechanistic studies on this U1 snRNP-specific splicing factor.