Genome-wide gene phylogeny of CIPK family in cassava and expression analysis of partial drought-induced genes.
Genome-wide gene phylogeny of CIPK family in cassava and expression analysis of partial drought-induced genes.
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DOI：
10.3389/fpls.2015.00914
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发表时间：
2015
影响因子：
5.6
通讯作者：
Peng M
中科院分区：
文献类型：
--
作者：
Hu W;Xia Z;Yan Y;Ding Z;Tie W;Wang L;Zou M;Wei Y;Lu C;Hou X;Wang W;Peng M
木薯是一种重要的粮食作物和潜在的生物燃料作物，对多种非生物胁迫具有耐受性。这些公差背后的机制目前还不太清楚。CBL相互作用蛋白激酶（CIPKs）在植物发育过程、激素信号转导和非生物胁迫应答中起着重要作用。然而，目前没有关于木薯中CPK家族的数据。本研究基于我们先前的基因组测序数据，从木薯基因组中共鉴定出25个CIPK基因。系统发育分析表明，25个MeCIPKs可分为4个亚家族，这是由外显子-内含子组织和保守蛋白基序的结构支持。对一个野生亚种和两个栽培品种的转录组学分析表明，大多数MeCIPKs在野生亚种和栽培品种之间在不同组织中或响应干旱胁迫时具有不同的表达模式。在拟南芥和木薯中发现了一些参与CIPK互作网络的直向同源基因。这些正交表位基因的互作网络和共表达模式揭示了CIPK网络控制的关键途径可能参与了不同木薯品种对干旱胁迫的差异反应。选择9个MeCIPK基因研究其对各种刺激的转录响应，结果显示所测试的MeCIPK基因对渗透、盐、冷、氧化胁迫和阿坝信号的综合响应。CIPK基因家族的鉴定和表达分析表明，CIPK基因是木薯发育过程中的重要组成部分，具有多种信号转导途径。本研究结果将有助于为CIPK基因家族的功能鉴定奠定基础，并为木薯非生物胁迫反应和信号转导提供更好的理解。
Cassava is an important food and potential biofuel crop that is tolerant to multiple abiotic stressors. The mechanisms underlying these tolerances are currently less known. CBL-interacting protein kinases (CIPKs) have been shown to play crucial roles in plant developmental processes, hormone signaling transduction, and in the response to abiotic stress. However, no data is currently available about the CPK family in cassava. In this study, a total of 25 CIPK genes were identified from cassava genome based on our previous genome sequencing data. Phylogenetic analysis suggested that 25 MeCIPKs could be classified into four subfamilies, which was supported by exon-intron organizations and the architectures of conserved protein motifs. Transcriptomic analysis of a wild subspecies and two cultivated varieties showed that most MeCIPKs had different expression patterns between wild subspecies and cultivatars in different tissues or in response to drought stress. Some orthologous genes involved in CIPK interaction networks were identified between Arabidopsis and cassava. The interaction networks and co-expression patterns of these orthologous genes revealed that the crucial pathways controlled by CIPK networks may be involved in the differential response to drought stress in different accessions of cassava. Nine MeCIPK genes were selected to investigate their transcriptional response to various stimuli and the results showed the comprehensive response of the tested MeCIPK genes to osmotic, salt, cold, oxidative stressors, and ABA signaling. The identification and expression analysis of CIPK family suggested that CIPK genes are important components of development and multiple signal transduction pathways in cassava. The findings of this study will help lay a foundation for the functional characterization of the CIPK gene family and provide an improved understanding of abiotic stress responses and signaling transduction in cassava.