The Propagation and Quantification of Two Emerging Oncolytic Viruses: Vesicular Stomatitis (VSV) and Zika (ZIKV).
The Propagation and Quantification of Two Emerging Oncolytic Viruses: Vesicular Stomatitis (VSV) and Zika (ZIKV).
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DOI：
10.1007/978-1-0716-0203-4_16
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发表时间：
2019-11
影响因子：
--
通讯作者：
R. Means;S. Roy;S. Katz
中科院分区：
文献类型：
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作者：
R. Means;S. Roy;S. Katz
基因工程的发展使研究人员和临床医生能够开始利用病毒来靶向并杀死癌细胞，要么通过直接裂解，要么通过招募抗病毒免疫反应。抗癌的两种强大病毒是单链RNA病毒、水泡性口炎病毒和寨卡病毒。在这里，我们描述了传播和滴定这两种病毒的方法。我们还描述了一种简单的细胞杀伤实验，以开始测试修饰的病毒增加对胶质母细胞瘤细胞的潜在杀伤力。
Developments in genetic engineering have allowed researchers and clinicians to begin harnessing viruses to target and kill cancer cells, either through direct lysis or through recruitment of antiviral immune responses. Two powerful viruses in the fight against cancer are the single-stranded RNA viruses vesicular stomatitis virus and Zika virus. Here, we describe methods to propagate and titer these two viruses. We also describe a simple cell-killing assay to begin testing modified viruses for increased potential killing of glioblastoma cells.