Listeria marthii sp nov., isolated from the natural environment, Finger Lakes National Forest
Listeria marthii sp nov., isolated from the natural environment, Finger Lakes National Forest
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DOI：
10.1099/ijs.0.014118-0
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发表时间：
2010-06-01
影响因子：
2.8
通讯作者：
Sauders, Brian D.
中科院分区：
文献类型：
--
作者：
Graves, Lewis M.;Helsel, Leta O.;Sauders, Brian D.
从从美国纽约芬格湖国家森林的自然环境中获得的土壤、静水和流水样品中分离出四种革兰氏阳性、能动、厌氧、非孢子形成杆菌的分离株（FSL S4-120（T）、FSL S4-696、FSL S4-710和FSL S4-965），它们与李斯特菌属的物种在表型上相似。这四个分离物彼此密切相关，并通过全基因组DNA杂交研究确定为同一物种（在55 ℃下>82%的相关性和在70 ℃下>76%的相关性，具有0.0-0.5%的分歧）。16 S rRNA基因序列分析证实了它们与单核细胞增生李斯特菌和无害李斯特菌的亲缘关系较近，与魏氏李斯特菌、L. seeligeri，L. ivanovii和L. grayi。对sigB、gap和sigB基因部分序列的系统发育分析表明，这些分离物与L.单核细胞增多症。这4个菌株与L.单核细胞增多症和L.通过DNA DNA杂交鉴定出无害李斯特菌，以证明它们是李斯特菌属的一个新物种。这四个分离株在AccuProbe检测中产生阳性反应，该检测据称对L。单核细胞增多症，不发酵L-鼠李糖，在血琼脂培养基上是非溶血性的，并且不包含L.单核细胞增生毒力基因岛根据其表型特征和与L.单核细胞增多症和L.无害李斯特菌（Listeria marthii sp. nov.）L. marthii是FSL S4-120（T）（=ATCC BAA-1595（T）=BEIR NR 9579（T）=CCUG 56148（T））。L.目前还没有发现马提氏病与人类或动物疾病有关。
Four isolates (FSL S4-120(T), FSL S4-696, FSL S4-710, and FSL S4-965) of Gram-positive, motile, facultatively anaerobic, non-spore-forming bacilli that were phenotypically similar to species of the genus Listeria were isolated from soil, standing water and flowing water samples obtained from the natural environment in the Finger Lakes National Forest, New York, USA. The four isolates were closely related to one another and were determined to be the same species by whole genome DNA DNA hybridization studies (>82% relatedness at 55 degrees C and >76% relatedness at 70 degrees C with 0.0-0.5% divergence). 16S rRNA gene sequence analysis confirmed their close phylogenetic relatedness to Listeria monocytogenes and Listeria innocua and more distant relatedness to Listeria welshimeri, L. seeligeri, L. ivanovii and L. grayi. Phylogenetic analysis of partial sequences for sigB, gap, and prs showed that these isolates form a well-supported sistergroup to L. monocytogenes. The four isolates were sufficiently different from L. monocytogenes and L. innocua by DNA DNA hybridization to warrant their designation as a new species of the genus Listeria. The four isolates yielded positive reactions in the AccuProbe test that is purported to be specific for L. monocytogenes, did not ferment L-rhamnose, were non-haemolytic on blood agar media, and did not contain a homologue of the L. monocytogenes virulence gene island. On the basis of their phenotypic characteristics and their genotypic distinctiveness from L. monocytogenes and L. innocua, the four isolates should be classified as a new species within the genus Listeria, for which the name Listeria marthii sp. nov. is proposed. The type strain of L. marthii is FSL S4-120(T) (=ATCC BAA-1595(T) =BEIR NR 9579(T) =CCUG 56148(T)). L. marthii has not been associated with human or animal disease at this time.