Monilinia species causing brown rot of peach in China.
Monilinia species causing brown rot of peach in China.
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引起我国桃褐腐病的褐腐病菌
DOI：
10.1371/journal.pone.0024990
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Luo CX
中科院分区：
文献类型：
--
作者：
Hu MJ;Cox KD;Schnabel G;Luo CX
本研究从我国多个省区采集了145份表现典型褐腐病症状的桃和油桃。26个单孢子分离物的子样本进行了遗传学和形态学表征，以确定物种。通过ITS区1和2、3-磷酸甘油醛脱氢酶（G3 PDH）和β-微管蛋白（TUB 2）的系统发育分析，发现了3个不同的串珠藻属种。这些种包括果生褐链霉菌（Monilinia fructicola）、木生褐链霉菌（Monilia mumecola）和一种以前未描述的云南褐链霉菌（Monilia yunnanensis sp. nov.）桃果生菌是我国桃的重要病原菌之一，桃果生菌M.在日本，mumecola最初只从梅果实中分离出来。科赫对M的假设。mumecola和M.结果表明，云南种对桃的致病性较强。ITS、G3 PDH和TUB 2序列的系统发育分析表明，M. yunnanensis与M. frustigena，一种在欧洲广泛流行的物种。有趣的是，有相当大的差异，外显子/内含子结构的细胞色素B（Cyt B）基因之间的两个物种。形态学特征，包括孢子大小，菌落形态，病斑生长速率和孢子形成，支持系统发育的证据，建议指定M。云南苔属（Yunnanensis）一新种。建立了一种新的多重PCR检测方法。云南褐腐病菌（Monilinia spp.）桃褐腐病在我国广泛发生。
In this study, 145 peaches and nectarines displaying typical brown rot symptoms were collected from multiple provinces in China. A subsample of 26 single-spore isolates were characterized phylogenetically and morphologically to ascertain species. Phylogenetic analysis of internal transcribed spacer (ITS) regions 1 and 2, glyceraldehyde-3-phosphate dehydrogenase (G3PDH), β-tubulin (TUB2) revealed the presence of three distinct Monilinia species. These species included Monilinia fructicola, Monilia mumecola, and a previously undescribed species designated Monilia yunnanensis sp. nov. While M. fructicola is a well-documented pathogen of Prunus persica in China, M. mumecola had primarily only been isolated from mume fruit in Japan. Koch's postulates for M. mumecola and M. yunnanensis were fulfilled confirming pathogenicity of the two species on peach. Phylogenetic analysis of ITS, G3PDH, and TUB2 sequences indicated that M. yunnanensis is most closely related to M. fructigena, a species widely prevalent in Europe. Interestingly, there were considerable differences in the exon/intron structure of the cytochrome b (Cyt b) gene between the two species. Morphological characteristics, including spore size, colony morphology, lesion growth rate, and sporulation, support the phylogenetic evidence suggesting the designation of M. yunnanensis as a new species. A new multiplex PCR method was developed to facilitate the detection of M. yunnanensis and differentiation of Monilinia spp. causing brown rot of peach in China.