Tolerance and resistance to a nematode challenge are not always mutually exclusive
Tolerance and resistance to a nematode challenge are not always mutually exclusive
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DOI：
10.1016/j.ijpara.2014.12.005
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发表时间：
2015-03-01
影响因子：
4
通讯作者：
Houdijk, Jos G. M.
中科院分区：
文献类型：
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作者：
Athanasiadou, Spiridoula;Tolossa, Ketema;Houdijk, Jos G. M.
耐受性的表现（宿主降低给定病原体水平的影响的能力）和抗药性（宿主清除病原体的能力）之间的关系被认为是一种拮抗作用。在这里，我们检验了以下假设：与耐药性较低的小鼠相比，对肠线虫具有更耐肠线虫的菌株的小鼠会降低耐受性。使用了三种近交菌株的小鼠：C57BL/6小鼠的特征是易感的，而BALB/C和NIH小鼠的特征是对Heligmosomoides Bakeri感染的抗性。每种菌株的小鼠要么用水中的250 1,3 H. bakeri（n = 10）的单剂量寄生，要么是用水伪造的（n = 10）。在整个实验中，定期记录体重，食物摄入和蠕虫卵输出。 42天P.I.确定了小鼠安乐死，并确定了器官的重量，结肠和蠕虫计数的卵。与NIH和BALB/C小鼠相比，C57BL16小鼠显示出明显更大的蠕虫卵输出（P <0.001），结肠中的卵（P <0.05）和雌性蠕虫繁殖力（P <0.05）。寄生的BALB/C小鼠在挑战后的前2周增长的C57BL/6小鼠增长了更多的C57BL/6小鼠（P = 0.05）。寄生虫显着增加了肝脏，脾脏，小肠和盲肠重量（p <0.001），但car体重量降低（p <0.01）。 NIH小鼠的平均每日体重增加和蠕虫数量呈正相关（p = 0.05）；但是，当将car体重量用作公差的量度时，这种关系被逆转。 BALB/C小鼠似乎并未遭受寄生虫的后果，所有动物的骨体重量相似。我们的假设是，与耐药性较低的菌株相比，对h. bakeri感染具有更耐药性的菌株被拒绝较低，因为这两种抗性菌株显示出可变的耐受性。因此，对肠线虫感染的耐受性和抗性并不总是相互排斥的。 （c）2015澳大利亚寄生虫学会，由Elsevier Ltd.出版。保留所有权利。
The relationship between the manifestations of tolerance (a host's ability to reduce the impact of a given level of pathogens) and resistance (a host's ability to clear pathogens) has been assumed to be an antagonistic one. Here we tested the hypothesis that mice from strains more resistant to intestinal nematodes will experience reduced tolerance compared with less resistant mice. Three inbred strains of mice were used: C57BL/6 mice have been characterised as susceptible, whereas BALB/c and NIH mice have been characterised as resistant to Heligmosomoides bakeri infection. Mice of each strain were either parasitised with a single dose of 250 1,3 H. bakeri (n = 10) in water or were sham-infected with water (n = 10). Body weight, food intake and worm egg output were recorded regularly throughout the experiment. Forty-two days p.i. mice were euthanised and organ weights, eggs in colon and worm counts were determined. C57BL16 mice showed significantly greater worm egg output (P < 0.001), eggs in colon (P < 0.05) and female worm fecundity (P < 0.05) compared with NIH and BALB/c mice. Parasitised BALB/c mice grew more whilst parasitised C57BL/6 mice grew less than their sham-infected counterparts during the first 2 weeks post-challenge (P = 0.05). Parasitism significantly increased liver, spleen, small intestine and caecum weights (P < 0.001) but reduced carcass weight (P < 0.01). Average daily weight gain and worm numbers were positively correlated in NIH mice (P = 0.05); however, the relationship was reversed when carcass weight was used as a measure for tolerance. BALB/c mice did not appear to suffer from the consequences of parasitism, with carcass weight similar in all animals. Our hypothesis that strains more resistant to the H. bakeri infection are less tolerant compared with less resistant strains is rejected, as the two resistant strains showed variable tolerance. Thus, tolerance and resistance to an intestinal nematode infection are not always mutually exclusive. (C) 2015 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.