Sex steroid receptors in lymphoid cells of human endometrium.
Sex steroid receptors in lymphoid cells of human endometrium.
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人子宫内膜淋巴细胞中的性类固醇受体。
DOI：
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发表时间：
1989
影响因子：
3.5
通讯作者：
P. Satyaswaroop
中科院分区：
文献类型：
--
作者：
S. Tabibzadeh;P. Satyaswaroop
性类固醇激素对靶组织的作用是通过雌激素和孕激素与特定核内蛋白、雌激素和孕激素受体（ER和PR）结合介导的。因此，在本报告中，作者研究了子宫内膜基质淋巴样细胞中ER和PR的存在，它们可能是子宫内膜中雌激素和黄体酮介导作用的潜在靶点。9例增殖性子宫内膜和10例分泌性子宫内膜中存在ER， 3例分泌性子宫内膜和1例增殖性子宫内膜中存在PR。ER和PR分别采用过氧化物酶-抗过氧化物酶（PAP）和亲和素-生物素-过氧化物酶复合物（ABC）方法，通过单克隆抗体定位到细胞核。然后用单克隆抗体对HLA-DR、白细胞共同抗原（LCA）、白细胞介素4 （CD3）和白细胞介素2受体分子进行修饰，并使用ABC染色程序对淋巴样细胞进行描绘。子宫内膜间质淋巴细胞聚集的一组细胞显示HLA-DR、LCA和Leu-4分子的膜性染色，有核ER。子宫内膜中IL-2受体阳性细胞少见，淋巴样聚集体中未见pr阳性细胞。此外，HLA-DR和ER在增生期的腺上皮和表面上皮中表达，在分泌后期的基底腺中偶有表达。子宫内膜淋巴细胞聚集物中er阳性淋巴细胞群的存在表明这些细胞可能是雌激素的靶细胞。
Sex steroid hormone action on target tissues is mediated through binding of estrogen and progesterone to specific intranuclear proteins, the estrogen and progesterone receptors (ER and PR). Therefore, in the present report the authors investigated for the presence of ER and PR in lymphoid cells of endometrial stroma that may serve as potential targets for estrogen- and progesterone-mediated effects in endometrium. The presence of ER was shown in nine proliferative and ten secretory endometria and the presence of PR in three secretory and one proliferative endometria. ER and PR were localized by monoclonal antibodies, to the nuclei of cells, with the use of, respectively, peroxidase-antiperoxidase (PAP) and avidin-biotin-peroxidase complex (ABC) methods. Lymphoid cells were then delineated by decoration of their plasma membranes with the use of monoclonal antibodies to HLA-DR, leukocyte common antigen (LCA), Leu-4 (CD3), and IL-2 receptor molecules with the use of an ABC staining procedure. A group of cells in the lymphoid aggregates in endometrial stroma showing membranous staining for HLA-DR, LCA, and Leu-4 molecules had nuclear ER. IL-2 receptor-positive cells were rare in endometrium, and no PR-positive cells were found in lymphoid aggregates. Furthermore, HLA-DR and ER were expressed in the glandular and surface epithelium in the proliferative phase and in occasional glands in the basalis in the late secretory phase. The presence of an ER-positive lymphoid cell population in endometrial lymphoid aggregates suggests that these cells may serve as target cells for estrogen.
雌激素受体定位于女性生殖道。
DOI：
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发表时间：
1986
期刊：
The American journal of pathology
影响因子：
--
作者：
Press,MF;Nousek-Goebl,NA;Bur,M;Greene,GL
通讯作者：
Greene,GL
人类自然杀伤细胞：生物学和病理学方面。
DOI：
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发表时间：
1984
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Trinchieri,G;Perussia,B
通讯作者：
Perussia,B