Extracellular oxidative enzyme production and PAH removal in soil by exploratory mycelium of white rot fungi
Extracellular oxidative enzyme production and PAH removal in soil by exploratory mycelium of white rot fungi
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DOI：
10.1023/a:1008324111558
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发表时间：
1999-06-01
期刊：
影响因子：
3.6
通讯作者：
Zadrazil, F
中科院分区：
文献类型：
--
作者：
Novotny, C;Erbanová, P;Zadrazil, F
选取糙皮侧耳、黄孢原毛革菌和杂色栓菌3种白色真菌菌株，在灭菌土壤中接种稻草，进行生长试验。各菌株的定殖率和菌丝密度值依次降低。三环和四环多环芳烃在50 ppm时对土壤中真菌的生长有一定的抑制作用。用50 mM琥珀酸-乳酸缓冲液（pH 4.5）提取的真菌锰过氧化物酶和漆酶（单位/克秸秆或土壤干重）的活性在秸秆中比在土壤中高5至20倍。糙皮侧耳和赤眼蜂每克干土的酶活性均高于野生侧耳。versicolor相似，相反，P. chrysosporium，在那里他们是非常低的。与通气对照相比，糙皮侧耳菌株在2个月内将蒽、芘和菲的水平分别降低了81- 87%、84-93%和41-64%。在蒽的降解过程中，所有糙皮侧耳菌株都积累了蒽醌。黄孢原毛平革菌和T.杂色土壤培养物则低得多。
Selected strains of three species of white rot fungi, Pleurotus ostreatus, Phanerochaete chrysosporium and Trametes versicolor, were grown in sterilized soil from straw inocula. The respective colonization rates and mycelium density values decreased in the above mentioned order. Three- and four-ringed PAHs at 50 ppm inhibited growth of fungi in soil to some extent. The activities of fungal MnP and laccase (units per g dry weight of straw or soil), extracted with 50 mM succinate-lactate buffer (pH 4.5), were 5 to 20-fold higher in straw compared to soil. The enzyme activities per g dry soil in P. ostreatus and T. versicolor were similar, in contrast to P. chrysosporium, where they were extremely low. Compared to the aerated controls, P. ostreatus strains reduced the levels of anthracene, pyrene and phenanthrene by 81-87%, 84-93% and 41-64% within 2 months, respectively. During degradation of anthracene, all P. ostreatus strains accumulated anthraquinone. PAH removal rates in P. chrysosporium and T. versicolor soil cultures were much lower.