Murine cortactin is phosphorylated in response to fibroblast growth factor-1 on tyrosine residues late in the G1 phase of the BALB/c 3T3 cell cycle.
Murine cortactin is phosphorylated in response to fibroblast growth factor-1 on tyrosine residues late in the G1 phase of the BALB/c 3T3 cell cycle.
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发表时间：
1993-11
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通讯作者：
X. Zhan;X. Hu;B. Hampton;W. Burgess;R. Friesel;T. Maciag
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作者：
X. Zhan;X. Hu;B. Hampton;W. Burgess;R. Friesel;T. Maciag
我们先前报道BALB/c 3 T3细胞需要长时间暴露于成纤维细胞生长因子（FGF）-1以刺激最大DNA合成，并且该事件与G1后期新蛋白质的酪氨酸磷酸化相关，包括称为p80/p85的蛋白质（Zhan，X.，Hu，X.，弗里茨河，和Maciag，T.（1993）J.Biol.Chem.268，9611-9620）。我们已纯化，测序，并克隆了cDNA编码p80/p85和报告，它是小鼠同源的鸡corneum基因和人造血特异性-1基因家族的成员。对FGF-1反应的m-皮质素-酪氨酸磷酸化的免疫化学分析显示了一种双相磷酸化模式，即弱的即刻早期和强的中期至晚期G1反应蛋白。由于鸡corneum基因最初是作为v-Src的底物分离的，FGF-1可能影响其他细胞相关酪氨酸激酶的酶活性，这些酪氨酸激酶利用p80/p85（corneum）作为多肽底物。
We have previously reported that BALB/c 3T3 cells require a prolonged exposure to fibroblast growth factor (FGF)-1 for the stimulation of maximal DNA synthesis, and this event correlates with the tyrosine phosphorylation of novel proteins late in G1 including a protein termed p80/p85 (Zhan, X., Hu, X., Friesel, R., and Maciag, T. (1993) J. Biol. Chem. 268, 9611-9620). We have purified, sequenced, and cloned the cDNA encoding p80/p85 and report that it is the murine homolog of the chicken cortactin gene and a member of the human hematopoietic specific-1 gene family. Immunochemical analysis of m-cortactin-tyrosine phosphorylation in response to FGF-1 demonstrates a biphasic phosphorylation pattern both as a weak immediate-early and strong mid to late G1 response protein. Because the chicken cortactin gene was originally isolated as a substrate for v-Src, FGF-1 may influence the enzymatic activity of other cell-associated tyrosine kinases which utilize p80/p85 (cortactin) as a polypeptide substrate.