Structural features of human tracheobronchial mucus glycoprotein.
Structural features of human tracheobronchial mucus glycoprotein.
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人气管支气管粘液糖蛋白的结构特征。
DOI：
10.1042/bj2220371
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Kaufman,B
中科院分区：
文献类型：
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作者：
Rose,MC;Voter,WA;Brown,CF;Kaufman,B
人气管支气管粘蛋白的铂影制备物的电子显微镜显示非常灵活的丝状结构，其经常发生在围绕致密核心样结构域的丝状物的复杂随机卷曲图案中。与核相关的丝占粘蛋白制剂质量的70-80%，其余为游离丝。在聚集时，分子形成大的交织网络，其与绵羊颌下粘蛋白聚集体的大量绳状结构特征完全不同[Rose，Voter，Sage，Brown &考夫曼（1984）J.Biol.Chem.259，3167-3172]。轻度超声处理导致气管支气管粘蛋白分子的广泛碎片化，并产生不同长度的短纤维，游离核心和一些与短纤维相关的核心。通过蛋白水解消化获得的粘蛋白糖肽片段是柔性的、无核心的细丝。链霉蛋白酶消化得到的糖肽比胰蛋白酶消化得到的糖肽短。人气管支气管粘蛋白的复杂结构明显不同于绵羊颌下和人卵巢囊肿粘蛋白的延伸细丝，但与Creeth和Knight [（1967）Biochem.J.105，1135-1145]基于流体动力学测量提出的粘蛋白的大致球形扩展模型一致。
Electron microscopy of platinum-shadowed preparations of human tracheobronchial mucins showed very flexible filamentous structures that frequently occurred in an intricate random-coiled pattern of filament(s) surrounding a dense core-like domain. The filament(s) associated with cores accounted for 70-80% of the mass of the mucin preparation, the remainder being accounted for by free filaments. On aggregation, the molecules formed a large interwoven network quite different from the massive rope-like structures characteristic of sheep submaxillary mucin aggregates [Rose, Voter, Sage, Brown & Kaufman (1984) J. Biol. Chem. 259, 3167-3172]. Mild sonication resulted in extensive fragmentation of the tracheobronchial mucin molecules and yielded short filaments of various lengths, free cores and some cores associated with short filaments. Mucin glycopeptide fragments obtained by proteolytic digestion were flexible, core-free, filaments. The glycopeptides obtained by Pronase digestion were shorter than those obtained by tryptic digestion. The intricate structures of human tracheobronchial mucin differ markedly from the extended filaments reported for sheep submaxillary and human ovarian-cyst mucins but agree with the roughly spherical expanded model proposed for mucins by Creeth & Knight [(1967) Biochem. J. 105, 1135-1145] on the basis of hydrodynamic measurements.
糖蛋白：其组成、结构和功能
DOI：
--
发表时间：
1972
期刊：
影响因子：
--
作者：
A. Gottschalk
通讯作者：
A. Gottschalk