Skeletal Repair in Rabbits Using a Novel Biomimetic Composite Based on Adipose-Derived Stem Cells Encapsulated in Collagen I Gel with PLGA-β-TCP Scaffold
Skeletal Repair in Rabbits Using a Novel Biomimetic Composite Based on Adipose-Derived Stem Cells Encapsulated in Collagen I Gel with PLGA-β-TCP Scaffold
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DOI：
10.1002/jor.20969
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发表时间：
2010-02-01
影响因子：
2.8
通讯作者：
Hu, Yun-Yu
中科院分区：
文献类型：
--
作者：
Hao, Wei;Pang, Long;Hu, Yun-Yu
在骨组织工程中，细胞在支架材料中的分布方式可能影响体内成骨。因此，我们构建了一种新型的仿生构建体，其基于包封在I型胶原凝胶中的兔脂肪源性干细胞（rASCs）与PLGA-β-TCP支架的组合（rASCs-CCL/PLGA-β-TCP，A组），rASCs和PLGA-β-TCP的组合B组（rASCs/PLGA-β-TCP）、I型胶原凝胶与PLGA-β-TCP的组合（COL/PLGA-β-TCP）、D组（PLGA-β-TCP支架）。复合材料植入到一个15毫米长的临界尺寸的节段性桡骨缺损。通过组织学、X线片、骨密度（BMD）和力学测试评估结果。24周后，A组髓腔再通，骨重建，成型完成，骨轮廓重塑顺利，支架完全降解。骨密度和力学性能与正常人相似。然而，B、C和D组的骨缺损仍未修复。此外，A组的支架降解率显著高于其他组。因此，增强了包裹在I型胶原凝胶中的rASCs与PLGA-β-TCP结合的体内成骨作用，并修复了骨缺损。我们希望这项研究为基于ASCs的骨组织工程提供新的见解。(C)2009骨科研究学会。出版社：Wiley Periodicals，Inc. J Orthop Res 28：252-257，2010
In bone tissue engineering, the cell distribution mode in the scaffold may affect in vivo osteogenesis. Therefore, we fabricated a novel biomimetic construct based on a combination of rabbit adipose-derived stem cells (rASCs) encapsulated in collagen I gel with a PLGA-beta-TCP scaffold (rASCs-CCL/PLGA-beta-TCP, group A), the combination of rASCs and PLGA-beta-TCP (rASCs/PLGA-beta-TCP, group B), the combination of collagen I gel and PLGA-beta-TCP (COL/PLGA-beta-TCP, group Q, and PLGA-beta-TCP scaffold (group D). The composites were implanted into a 15-mm length critical-sized segmental radial defect. The results were assessed by histology, radiographs, bone mineral density (BMD), and mechanical testing. After 24 weeks, the medullary cavity recanalized, bone was rebuilt, and molding finished, the bone contour remodeled smoothly and the scaffold degraded completely in group A. The BMDs and mechanical properties were similar to normal. However, the bone defect remained unrepaired in groups B, C, and D. Moreover, the scaffold degradation rate in group A was significantly higher than the other groups. Thus, enhanced in vivo osteogenesis of rASCs wrapped in collagen I gel combined with PLGA-beta-TCP was achieved, and the bone defect was repaired. We hope this study provides new insights into ASCs-based bone tissue engineering. (C) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:252-257, 2010