Characterization of serracin P, a phage-tail-like bacteriocin, and its activity against Erwinia amylovora, the fire blight pathogen
Characterization of serracin P, a phage-tail-like bacteriocin, and its activity against Erwinia amylovora, the fire blight pathogen
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DOI：
10.1128/aem.68.11.5704-5710.2002
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发表时间：
2002-11-01
影响因子：
4.4
通讯作者：
Thonart, P
中科院分区：
文献类型：
--
作者：
Jabrane, A;Sabri, A;Thonart, P
普雷氏沙雷氏菌 V 培养物上清液对梨火疫病菌（苹果和梨树最严重的细菌性疾病火疫病的病原体）的多种致病菌株具有活性，并且对肺炎克雷伯氏菌、液化沙雷氏菌、粘质沙雷氏菌和荧光假单胞菌具有活性。用丝裂霉素 C 诱导后，这种活性显着增加。从 S. plymithicum V 的诱导培养物上清液中纯化出一种噬菌体尾样细菌素，称为 Serracin P。它被发现是唯一对敏感菌株具有抗菌活性的化合物。 Serracin P 的两个主要亚基（23 和 43 kDa）的 N 端氨基酸序列分析显示，其与肠沙门氏菌的 Fels-2 前噬菌体、大肠杆菌噬菌体 P2 和 168、铜绿假单胞菌的 phiCTX 前噬菌体以及鼠疫耶尔森氏菌的前噬菌体具有高度同源性。这强烈表明 Serracin P 和这些噬菌体具有共同的祖先。
Serratia plymithicum V culture supernatant displayed activity against many pathogenic strains of Erwinia amylovora, the causal agent of the most serious bacterial disease of apple and pear trees, fire blight, and against Klebsiella pneumoniae, Serratia liquefaciens, Serratia marcescens, and Pseudomonas fluorescens. This activity increased significantly upon induction with mitomycin C. A phage-tail-like bacteriocin, named serracin P, was purified from an induced culture supernatant of S. plymithicum V. It was found to be the only compound involved in the antibacterial activity against sensitive strains. The N-terminal amino acid sequence analysis of the two major subunits (23 and 43 kDa) of serracin P revealed high homology with the Fels-2 prophage of Salmonella enterica, the coliphages P2 and 168, the phiCTX prophage of Pseudomonas aeruginosa, and a prophage of Yersinia pestis. This strongly suggests a common ancestry for serracin P and these bacteriophages.