snoRNAs associate with mRNA 3' processing complex: New wine in old bottles.
snoRNAs associate with mRNA 3' processing complex: New wine in old bottles.
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DOI：
10.1080/15476286.2017.1416278
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发表时间：
2018
期刊：
影响因子：
4.1
通讯作者：
Yao Chengguo
中科院分区：
文献类型：
--
作者：
Shi Junjie;Huang Chunliu;Huang Shanshan;Yao Chengguo
3′末端加工是所有真核RNA成熟所必需的。目前的模型表明，典型的mRNA 3′加工仅在称为mRNA 3′加工复合物的蛋白质复合物内进行。在最近的一项研究中，通过使用基于RNA-生物素的下拉分析和高通量测序，我们报道了一个小核仁RNA（snoRNA）的子集与这种大分子机制物理相关。通过对其中一种snoRNA（SNORD 50 A）的详细分析，我们发现非编码RNA（如snoRNA）可能在mRNA的3′端加工过程中起调控作用。我们的研究结果为mRNA 3′加工的调控机制和snoRNA的非经典功能提供了新的见解。
3′ end processing is required for the maturation of all eukaryotic RNAs. Current model suggests that canonical mRNA 3′ processing is carried out exclusively within a protein complex termed mRNA 3′ processing complex. In a recent study, by using RNA-biotin based pull-down assay and high-throughput sequencing, we reported that a subset of small nucleolar RNAs (snoRNAs) were physically associated with this macromolecular machinery. Through detailed characterization of one of these snoRNAs, SNORD50A, we revealed that non-coding RNA, such as snoRNA, may play a regulatory role in mRNA 3′ processing. Our results provided novel insight into both the regulatory mechanism of mRNA 3′ processing and the non-canonical functions of snoRNAs.