Large Thermal Conductivity Differences between the Crystalline and Vitrified States of DMSO with Applications to Cryopreservation.
Large Thermal Conductivity Differences between the Crystalline and Vitrified States of DMSO with Applications to Cryopreservation.
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DOI：
10.1371/journal.pone.0125862
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Rabin Y
中科院分区：
文献类型：
--
作者：
Ehrlich LE;Feig JS;Schiffres SN;Malen JA;Rabin Y
在这项研究中，使用瞬态热线技术，二甲基亚砜（DMSO）溶液的热导率测量，其中DMSO是许多冷冻保护剂（CPA）鸡尾酒的关键成分。低温保护剂的热性能的表征是必不可少的低温保存过程的分析，无论是在评估实验数据或设计新的协议。还介绍了纯水、冰和甘油的导热系数的参考测量值。热导率测量设置被集成到扫描低温宏观镜装置的实验阶段中，这有助于将测量数据与物理事件的可视化相关联。在-180 ° C和25°C的温度范围内，对2 M和10 M的DMSO浓度范围进行热导率测量。玻璃化样品的热导率随着温度的降低而降低，而结晶样品的热导率随着温度的降低而增加。这些不同的行为导致在-180 ° C下的热导率差异高达十倍。这种巨大的差异可以极大地影响低温保存过程中的传热，因此它们的定量对低温生物学至关重要。
Thermal conductivity of dimethyl-sulfoxide (DMSO) solution is measured in this study using a transient hot wire technique, where DMSO is a key ingredient in many cryoprotective agent (CPA) cocktails. Characterization of thermal properties of cryoprotective agents is essential to the analysis of cryopreservation processes, either when evaluating experimental data or for the design of new protocols. Also presented are reference measurements of thermal conductivity for pure water ice and glycerol. The thermal conductivity measurement setup is integrated into the experimentation stage of a scanning cryomacroscope apparatus, which facilitates the correlation of measured data with visualization of physical events. Thermal conductivity measurements were conducted for a DMSO concentration range of 2M and 10M, in a temperature range of -180°C and 25°C. Vitrified samples showed decreased thermal conductivity with decreasing temperature, while crystalline samples showed increased thermal conductivity with decreasing temperature. These different behaviors result in up to a tenfold difference in thermal conductivity at -180°C. Such dramatic differences can drastically impact heat transfer during cryopreservation and their quantification is therefore critical to cryobiology.
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DOI：
10.1016/0378-4363(76)90203-5
发表时间：
1976-01-01
期刊：
PHYSICA B & C
影响因子：
--
作者：
HEALY, JJ;DEGROOT, JJ;KESTIN, J
通讯作者：
KESTIN, J
影响因子：
2.1
作者：
CAHILL, DG;POHL, RO
通讯作者：
POHL, RO
影响因子：
--
作者：
Devireddy, RV;Smith, DJ;Bischof, JC
通讯作者：
Bischof, JC
影响因子：
2.7
作者：
MERYMAN, HT
通讯作者：
MERYMAN, HT
影响因子：
--
作者：
Steif, Paul S.;Palastro, Matthew C.;Rabin, Yoed
通讯作者：
Rabin, Yoed