Effects of 2,4-Dichlorophenoxyacetic Acid on Cucumber Fruit Development and Metabolism
Effects of 2,4-Dichlorophenoxyacetic Acid on Cucumber Fruit Development and Metabolism
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2,4-二氯苯氧基乙酸对黄瓜果实发育和代谢的影响
DOI：
10.3390/ijms20051126
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发表时间：
2019-03-01
影响因子：
5.6
通讯作者：
Yang, Guiling
中科院分区：
文献类型：
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作者：
Hu, Chaoyang;Zhao, Huiyu;Yang, Guiling
生长素类化合物2，4-二氯苯氧乙酸（2，4-D）作为植物生长调节剂在黄瓜生产中被广泛应用，但其对果实发育和代谢的影响尚未得到评价。本研究记录了2，4-D处理组和未处理对照组黄瓜果实的表型，并采用基于UPLC-qTOF-MS的标准化非靶向代谢组学方法，分析了不同采样时间点黄瓜果实不同节段的代谢组。4-D促进了黄瓜果长的早期生长，但对最终果长的影响不显著，且黄瓜果实顶部有鲜花。2，4-D处理还影响了黄瓜果实的代谢组，导致开花后4天（10天）花柱末端发生显著变化。代谢产物主要涉及蛋氨酸代谢、柠檬酸循环和黄酮代谢途径。在采收期，2，4-D处理显著降低了类黄酮和肉桂酸衍生物的水平，而增加了一些氨基酸的水平。综上所述，外源2，4-D能显著改变黄瓜果实的表型和代谢。这些研究结果将有助于探讨2，4-D处理对黄瓜果实表型的影响机制，以及评价2，4-D处理对黄瓜果实营养品质的影响。
The auxin-like compound 2,4-dichlorophenoxyacetic acid (2,4-D) has been widely used as a plant growth regulator in cucumber fruit production; however, its influence on fruit development and metabolism has not been evaluated. In this study, the phenotype of cucumber fruits in both 2,4-D treatment and non-treatment control groups were recorded, and the metabolome of different segments of cucumber fruit at various sampling time points were profiled by a standardized non-targeted metabolomics method based on UPLC-qTOF-MS. The application of 2,4-D increased the early growth rate of the fruit length but had no significant effect on the final fruit length, and produced cucumber fruits with fresh flowers at the top. The 2,4-D treatment also affected the cucumber fruit metabolome, causing significant changes in the stylar end at 4 days after flowering (DAF). The significantly changed metabolites were mainly involved in methionine metabolism, the citric acid cycle and flavonoid metabolism pathways. At the harvest stage, 2,4–D treatment significantly decreased the levels of flavonoids and cinnamic acid derivatives while increased the levels of some of the amino acids. In summary, exogenous application of 2,4-D can greatly alter the phenotype and metabolism of cucumber fruit. These findings will assist in exploring the mechanisms of how 2,4-D treatment changes the fruit phenotype and evaluating the influence of 2,4-D treatment on the nutritional qualities of cucumber fruit.