Development and Characterization of Monoclonal Antibodies Directed against the Nucleoprotein of Heartland Virus
Development and Characterization of Monoclonal Antibodies Directed against the Nucleoprotein of Heartland Virus
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DOI：
10.4269/ajtmh.15-0473
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发表时间：
2015-12-01
影响因子：
3.3
通讯作者：
Branlt, Aaron C.
中科院分区：
文献类型：
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作者：
Calvert, Amanda E.;Branlt, Aaron C.
Heartland 病毒 (HRTV) 是一种白蛉病毒，于 2009 年首次从密苏里州两名农民身上分离出来，据推测，该病毒可通过受感染的美洲钝蜱叮咬传播给人类。它与来自中国的严重发热伴血小板减少综合征病毒（SFTSV）密切相关，这是另一种以前未被识别的白蛉病毒，随后与数百例人类严重疾病相关。为了扩大检测 HRTV 感染的诊断能力，使用 HRTV 接种的 AG129 α/β 和 γ 干扰素受体缺陷小鼠的脾细胞开发了 20 个分泌抗 HRTV 鼠单克隆抗体 (1VIAb) 的杂交瘤克隆。本文对这些 MAb 中的 9 种进行了表征，以便将其纳入未来 HRTV 诊断测定的开发中。发现所有开发的单克隆抗体都对 HRTV 核衣壳蛋白上的线性表位具有非中和性和反应性。发现 MAb 2AF11 与 SFTSV 存在交叉反应。
Heartland virus (HRTV), a phlebovirus first isolated from two Missouri farmers in 2009, has been proposed to be transmitted to humans by the bite of infected Amblyomma americanum ticks. It is closely related to severe fever with thrombocytopenia syndrome virus (SFTSV) from China, another previously unrecognized phlebovirus that has subsequently been associated with hundreds of cases of severe disease in humans. To expand diagnostic capacity to detect HRTV infections, 20 hybridoma clones secreting anti-HRTV murine monoclonal antibodies (1VIAbs) were developed using splenocytes from HRTV-inoculated AG129 alpha/beta and gamma interferon receptor deficient mice. Nine of these MAbs were characterized herein for inclusion in future HRTV diagnostic assay development. All of the MAbs developed were found to be non-neutralizing and reactive to linear epitopes on HRTV nucleocapsid protein. MAb 2AF11 was found to be cross-reactive with SFTSV.