Development of eosinophilic airway inflammation and airway hyperresponsiveness requires interleutkin-5 but not immunoglobulin E or B lymphocytes
Development of eosinophilic airway inflammation and airway hyperresponsiveness requires interleutkin-5 but not immunoglobulin E or B lymphocytes
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DOI：
10.1165/ajrcmb.21.4.3659
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发表时间：
1999-10-01
影响因子：
6.4
通讯作者：
Gelfand, EW
中科院分区：
文献类型：
--
作者：
Hamelmann, E;Takeda, K;Gelfand, EW
我们以前定义了B细胞和过敏原特异性免疫球蛋白在过敏性致敏、气道炎症和气道高反应性（AHR）发展中的作用，使用10-d方案，其中过敏原暴露仅通过气道发生，无佐剂。在本方案中，正常和B细胞缺陷（μ Mt（-/-））小鼠腹腔内致敏卵清蛋白（OVA），并通过气道用OVA激发，以检查本方案对AHR的要求。T细胞活化（抗原特异性增殖反应和Th 2型细胞因子产生）和嗜酸性粒细胞浸润的气道支气管周围区域，嗜酸性粒细胞活化和脱粒的迹象，发生在两个实验组。与10天的协议，增加体内气道反应乙酰甲胆碱和体外气管平滑肌反应电场刺激观察正常和B细胞缺陷小鼠，这些反应被抑制抗白细胞介素（IL）-5管理前气道挑战。这些数据表明，IL-5，而不是B细胞或过敏原特异性IgE，是嗜酸性粒细胞气道浸润和过敏原/明矾致敏和过敏原反复气道激发后AHR发展所必需的。这些结果强调，使用不同的致敏和挑战协议可以影响AHR发展的要求。
We previously defined a role for B cells and allergen-specific immunoglobulins in the development of allergic sensitization, airway inflammation, and airway hyperresponsiveness (AHR), using a 10-d protocol in which allergen exposure occurred exclusively via the airways, without adjuvant. In the present protocol, normal and B-cell-deficient (mu Mt(-/-)) mice were sensitized intraperitoneally to ovalbumin (OVA) and challenged with OVA via the airways in order to examine the requirements for AHR with this protocol. T-cell activation (antigen-specific proliferative responses and Th2-type cytokine production) and eosinophil infiltration in the peribronchial regions of the airways, with signs of eosinophil activation and degranulation, occurred in both experimental groups. In contrast to the 10-d protocol, increased in vivo airway responsiveness to methacholine and in vitro tracheal smooth-muscle responses to electrical field stimulation were observed in both normal and B-cell-deficient mice, and these responses were inhibited by anti-interleukin (IL)-5 administration before airway challenge. These data show that IL-5, but not B cells or allergen-specific IgE, are required for eosinophil airway infiltration and the development of AHR following allergen/alum sensitization and repeated airway challenge with allergen. These results emphasize that the use of different sensitization and challenge protocols can influence the requirements for development of AHR.