A Recognition Model of tRNASer by Seryl-tRNA Synthetase in E. coli.
A Recognition Model of tRNASer by Seryl-tRNA Synthetase in E. coli.
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大肠杆菌 Seryl-tRNA 合成酶对 tRNASer 的识别模型。
DOI：
10.1002/chin.199208310
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
M. Shimizu
中科院分区：
文献类型：
--
作者：
H. Asahara;H. Himeno;M. Shimizu
大肠杆菌丝氨酰-tRNA合成酶(SerRs)是一种二聚体酶，其亚基由两个结构域组成，即一个球状结构域和一个长的反平行的α-螺旋卷曲结构域。利用T7转录系统对tRNA Ser的识别位点进行了搜索，提出了一种新的识别机制模型，即一个亚基上有一个与tRNA Ser长臂相互作用的长线圈状结构域，另一个亚基上有一个与tRNA Ser 3‘端相互作用的球状结构域
E. coli seryl-tRNA synthetase (SerRS) is a dimeric enzyme, having a subunit composed of two domains, i.e., a globular domain that includes a putative active site, and a long antiparallel α-helical coiled coil domain. The recognition sites on tRNA Ser by SerRS were searched using the T7 transcription system, and a new model of the recognition mechanism was proposed, consisting of a long coil shaped domain on one subunit which interacts with the long extra arm of tRNA Ser , and a globular domain of the other subunit which interacts with the 3' end of tRNA Ser