Airborne bacteria and antibiotic resistance genes in hospital rooms
Airborne bacteria and antibiotic resistance genes in hospital rooms
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DOI：
10.1007/s10453-010-9155-1
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发表时间：
2010-09-01
期刊：
影响因子：
2
通讯作者：
Duchaine, Caroline
中科院分区：
文献类型：
--
作者：
Gilbert, Yan;Veillette, Marc;Duchaine, Caroline
在肺科病房对最近入住的医院病房中生物气溶胶的微生物生物多样性进行了评估。环境样品和分离株也筛选抗生素耐药基因。还研究了水槽排水沟的生物膜，以评估水槽排水沟是否构成这种环境中生物气溶胶的潜在来源以及机会细菌和抗生素耐药基因的储存库。嗜麦芽窄食单胞菌是迄今为止从生物膜中分离出的最常见的微生物，其次是阴沟肠杆菌。空气中细菌浓度范围为14至74 CFU m（-3），真菌范围为50至600 CFU m（-3）。气溶胶中的生物膜细菌数量超过了与人体皮肤植物群相关的微生物。尽管如此，从空气样本中回收的这些物质浓度很低。所有病房空气样本均检出红霉素耐药基因，零星检出四环素耐药基因。在一个单一的排水管中发现了抗生素耐药性基因，这表明空气样本中DNA提取物中存在的基因不是从水槽排水管中雾化出来的，而是来自未知来源。在这项研究中获得的结果表明，从水槽排水细菌没有雾化在显着的浓度。它们仍然是一个令人关切的问题，因为它们的存在有空中传播的风险。
The microbial biodiversity of bioaerosols in recently occupied hospital rooms was assessed in a pulmonology unit. Environmental samples and isolates were also screened for antibiotics resistance genes. Biofilms from sink drains were also studied to evaluate whether sink drains constitute a potential source of bioaerosols in this environment and a reservoir for opportunistic bacteria and antibiotic resistance genes. Stenotrophomonas maltophilia was by far the most frequently isolated microorganisms from the biofilm, followed by Enterobacter cloacae. Airborne bacterial concentration ranged from 14 to 74 CFU m(-3) and fungi ranged from 50 to 600 CFU m(-3). Biofilm bacteria were outnumbered in aerosols by microorganisms affiliated with human skin flora. Nonetheless, they were recovered from air samples in low concentrations. Erythromycin resistance genes were detected in all air samples collected from hospital rooms, and tetracycline resistance genes were detected sporadically. Antibiotic resistance genes were found in a single drain suggesting that genes present in DNA extracts from air samples were not aerosolized from sink drains, but rather from an unknown source. Results obtained in this study suggest that bacteria from sink drains were not aerosolized in significant concentration. They still remain a concern because of the risk of aerial transmission associated with their presence.