Design and synthesis of nonionic copolypeptide hydrogels with reversible thermoresponsive and tunable physical properties.
Design and synthesis of nonionic copolypeptide hydrogels with reversible thermoresponsive and tunable physical properties.
复制标题
具有可逆的热响应和可调的物理特性的非离子共聚型水凝胶的设计和合成。
DOI：
10.1021/acs.biomac.5b00124
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发表时间：
2015-04-13
影响因子：
6.2
通讯作者：
Deming TJ
中科院分区：
文献类型：
--
作者：
Zhang S;Alvarez DJ;Sofroniew MV;Deming TJ
基于多肽的配方在温度变化时经历液体到水凝胶的转变已经成为理想的目标，因为它们可以与细胞混合或作为液体注射到组织中，并随后转化为刚性支架或仓库。用合成多肽制备这种材料一直具有挑战性，特别是当需要可逆凝胶和可调物理性质时。在此，我们设计并制备了新的非离子二嵌段共肽水凝胶（DCH），命名为DCHEO，并进一步在DCHEO中加入共肽结构域，得到了前所未有的热响应性DCH，命名为DCHT。虽然以前的尝试制备非离子型水凝胶完全由合成多肽组成是不成功的，我们的设计产生的材料具有高度可逆的热转变和可调的性质。非离子型、热响应性DCHT被发现支持悬浮间充质干细胞的体外生存能力，并且能够溶解并提供亲水和疏水分子的长时间释放。DCHT液体在室温下的粘度、DCHT水凝胶在高温下的刚度以及DCHT液体到水凝胶的转变温度本身的独立分子调节进一步证明了这些材料的多功能性。
Polypeptide based formulations that undergo liquid to hydrogel transitions upon change in temperature have become desirable targets since they can be mixed with cells or injected into tissues as liquids, and subsequently transform into rigid scaffolds or depots. Such materials have been challenging to prepare using synthetic polypeptides, especially when reversible gelation and tunable physical properties are desired. Here, we designed and prepared new non-ionic diblock copolypeptide hydrogels (DCH) named DCHEO, and also further incorporated copolypeptide domains into DCHEO to yield unprecedented thermoresponsive DCH, named DCHT. Although previous attempts to prepare non-ionic hydrogels composed solely of synthetic polypeptides have been unsuccessful, our designs yielded materials with highly reversible thermal transitions and tunable properties. Non-ionic, thermoresponsive DCHT were found to support the viability of suspended mesenchymal stem cells in vitro, and were able to dissolve and provide prolonged release of both hydrophilic and hydrophobic molecules. The versatility of these materials was further demonstrated by the independent molecular tuning of DCHT liquid viscosity at room temperature, DCHT hydrogel stiffness at elevated temperature, as well as the DCHT liquid to hydrogel transition temperature itself.