Reduced expression of the NADPH oxidase NOX4 is a hallmark of adipocyte differentiation
Reduced expression of the NADPH oxidase NOX4 is a hallmark of adipocyte differentiation
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DOI：
10.1016/j.bbamcr.2007.03.003
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发表时间：
2007-07-01
影响因子：
5.1
通讯作者：
Szanto, Ildiko
中科院分区：
文献类型：
--
作者：
Mouche, Sarah;Ben Mkaddem, Sanae;Szanto, Ildiko
脂肪细胞分化是一个复杂的过程，受胰岛素和活性氧（ROS）的产生等因素的调节。NOX 4是一种产生ROS的NADPH氧化酶，在3 T3 L1脂肪细胞中介导胰岛素的作用。在本文中，我们表明，NOX 4在白色和]）城市前脂肪细胞中均以高水平表达，并且分化为脂肪细胞导致其NOX 4 mRNA含量降低。这些体外结果在体内得到证实，证明在完整的脂肪组织中，大多数表达NOX 4的细胞位于含有基质/血管部分的前脂肪细胞内，而不是由成熟脂肪细胞组成的部分。与这些观察结果一致，来自不同啮齿动物胰岛素抵抗模型的脂肪中的N 0X 4 mRNA的定量表明，N 0X 4表达的改变反映了脂肪细胞/间质部分的比率的变化。总之，我们揭示了NOX 4 mRNA含量的降低是脂肪细胞分化的标志，并且在整个脂肪组织中测量的NOX 4表达并不是胰岛素作用完整或受损的明确指标。（0 2007 Elsevier BN. All rights reserved.
Adipocyte differentiation is a complex process regulated among other factors by insulin and the production of reactive oxygen species (ROS). NOX4 is a ROS generating NADPH oxidase enzyme mediating insulin's action in 3T3L1 adipocytes. In the present paper we show that NOX4 is expressed at high levels both in white and])town preadipocytes and that differentiation into adipocytes results in a decrease in their NOX4 mRNA content. These in vitro results were confirmed in vivo by demonstrating that in intact adipose tissue the majority of NOX4 expressing cells are localized within the preadipocyte containing stromal/vascular fraction, rather than in the portion consisting of mature adipocytes. In line with these observations, quantification of NOX4 mRNA in fat derived from different rodent models of insulin resistance indicated that alteration in NOX4 expression reflects changes in the ratio of adipocyte/interstitial fractions. In conclusion, we reveal that decreased NOX4 mRNA content is a hallmark of adipocyte differentiation and that NOX4 expression measured in whole adipose tissue is not an unequivocal indicator of intact or impaired insulin action. (0 2007 Elsevier BN. All rights reserved.