Genomic Analysis of an Ascomycete Fungus from the Rice Planthopper Reveals How It Adapts to an Endosymbiotic Lifestyle.
Genomic Analysis of an Ascomycete Fungus from the Rice Planthopper Reveals How It Adapts to an Endosymbiotic Lifestyle.
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稻飞虱子囊菌的基因组分析揭示了它如何适应内共生生活方式
DOI：
10.1093/gbe/evv169
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发表时间：
2015-09-02
影响因子：
3.3
通讯作者：
Zhang CX
中科院分区：
文献类型：
--
作者：
Fan HW;Noda H;Xie HQ;Suetsugu Y;Zhu QH;Zhang CX
许多吸汁昆虫体内都含有内共生体，这些内共生体被认为在寄主的发育过程中起着重要作用。褐飞虱（Nilaparvata lugens（Stål））是水稻上最重要的害虫之一，其体内存在一种不能在体外培养的专性酵母样共生体（YLS）。这YLS的基因组信息将有助于更好地了解其演变。在这项研究中，我们使用454和Illumina方法对YLS进行了基因组测序，生成了一个基因组草图，该草图显示出比大多数子囊菌真菌略小的基因组大小和相对较高的GC含量。对YLS的基因组分析表明，YLS与昆虫病原菌有着密切的关系。我们分析了YLS特异性基因和在YLS进化过程中可能发生变化的基因类别。YLS中交配型位点的缺失揭示了真核生物共生体的进化。这一信息的YLS基因组提供了一个有用的指导，进一步了解内共生协会半翅目和共生替换古老的细菌与多功能的YLS似乎是一个成功的变化。
A number of sap-sucking insects harbor endosymbionts, which are thought to play an important role in the development of their hosts. One of the most important rice pests, the brown planthopper (BPH), Nilaparvata lugens (Stål), harbors an obligatory yeast-like symbiont (YLS) that cannot be cultured in vitro. Genomic information on this YLS would be useful to better understand its evolution. In this study, we performed genome sequencing of the YLS using both 454 and Illumina approaches, generating a draft genome that shows a slightly smaller genome size and relatively higher GC content than most ascomycete fungi. A phylogenomic analysis of the YLS supported its close relationship with insect pathogens. We analyzed YLS-specific genes and the categories of genes that are likely to have changed in the YLS during its evolution. The loss of mating type locus demonstrated in the YLS sheds light on the evolution of eukaryotic symbionts. This information about the YLS genome provides a helpful guide for further understanding endosymbiotic associations in hemiptera and the symbiotic replacement of ancient bacteria with a multifunctional YLS seems to have been a successful change.