Development and stability of bacteriocin resistance in Campylobacter spp.
Development and stability of bacteriocin resistance in Campylobacter spp.
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DOI：
10.1111/j.1365-2672.2011.05163.x
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发表时间：
2011-12-01
影响因子：
4
通讯作者：
Lin, J.
中科院分区：
文献类型：
--
作者：
Hoang, K. V.;Stern, N. J.;Lin, J.
目的：从鸡共生细菌中鉴定出的几种细菌素（BCN）可显着减少家禽中弯曲杆菌的定植，并用于在农场控制这种重要的食源性人类病原体。最近的一项研究表明，空肠弯曲杆菌很难在体外产生 BCN 耐药性。在这项研究中，检查了弯曲杆菌BCN抗性的体内发展和稳定性。方法和结果：感染Camp的鸡。空肠弯曲杆菌NCTC 11168以5 mg kg(-1)体重day(-1)的剂量连续三天经口灌胃BCN E-760，在处理的鸡中筛选出BCN抗性(BCNr)突变体。然而，与亲本菌株（MIC = 0 5 mg l(-1)）相比，所有体内选择的突变体仅表现出低水平的BCN抗性（MIC = 2-8 mg l(-1)）。 BCNr 突变体的 CmeABC 外排泵失活导致对 BCN 的敏感性增加（MIC 降低 8-32 倍）。使用体外和体内系统检查了三种不同的 BCNr 弯曲杆菌菌株（体外或体内衍生的）BCN 耐药性的稳定性。在没有BCN选择压力的情况下，这些菌株的低水平BCN抗性在体外或体内不稳定。结论：使用BCN E-760仅选择低水平BCNr Camp。研究的意义和影响：该研究为未来抗弯曲菌BCN在动物中的实际应用的风险评估提供了有用的信息。
Aims: Several bacteriocins (BCNs) that were identified from chicken commensal bacteria dramatically reduced Campylobacter colonization in poultry and are being directed toward on-farm control of this important foodborne human pathogen. A recent study has shown that BCN resistance in Campylobacter jejuni is very difficult to develop in vitro. In this study, in vivo development and stability of BCN resistance in Campylobacter was examined.Methods and Results: Chickens infected with Camp. jejuni NCTC 11168 were treated with BCN E-760 at the dose of 5 mg kg(-1) body weight day(-1) via oral gavages for three consecutive days, which selected BCN-resistant (BCNr) mutants in the treated birds. However, all the in vivo-selected mutants only displayed low levels of resistance to BCN (MIC = 2-8 mg l(-1)) when compared to parent strain (MIC = 0 5 mg l(-1)). Inactivation of CmeABC efflux pump of the BCNr mutants led to increased susceptibility to BCN (8-32 fold MIC reduction). Three different BCNr Campylobacter strains (in vitro-or in vivo-derived) were examined for the stability of BCN resistance using both in vitro and in vivo systems. The low level of BCN resistance in these strains was not stable in vitro or in vivo in the absence of BCN selection pressure.Conclusions: Usage of BCN E-760 only selected low-level BCNr Camp. jejuni mutants in vivo, and the low-level BCN resistance was not stable in vitro and in vivo.Significance and Impact of the Study: The study provides helpful information for risk assessment of the future practical application of the anti-Campylobacter BCNs in animals.