Effects of tetramethylpyrazine on nitric oxide/cGMP signaling after cerebral vasospasm in rabbits
Effects of tetramethylpyrazine on nitric oxide/cGMP signaling after cerebral vasospasm in rabbits
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DOI：
10.1016/j.brainres.2010.09.011
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发表时间：
2010-11-18
期刊：
影响因子：
2.9
通讯作者：
Liu, Xiangzhen
中科院分区：
文献类型：
--
作者：
Shao, Zhengkai;Li, Jingwen;Liu, Xiangzhen
川芎嗪（Tetramethylpyrazine， TMP）是川芎的主要成分之一，具有血管松弛作用。蛛网膜下腔出血（SAH）后脑血管痉挛（CVS）与高死亡率和发病率相关。在这里，我们评估了TMP在CVS模型中的作用，并试图确定其潜在的作用机制。通过大池注射自体血建立家兔SAH模型。采用经颅多普勒（TCD）和计算机断层血管造影（CTA）测量脑血流量和动脉直径。western blot检测基底动脉eNOS和PDE-V的表达。测定血浆和脑脊液中一氧化氮（NO）和内皮细胞内Ca2+水平。SAH动物的BAs直径明显减小，血流速度明显加快(P
Tetramethylpyrazine (TMP), an ingredient of Chinese herbal Szechwan lovage rhizome, shows vasorelaxant effect. Cerebral vasospasm (CVS) after subarachnoid hemorrhage (SAH) is associated with high mortality and morbidity. Here, we evaluated the effect of TMP in a model of CVS and sought to identify the underlying mechanisms of action. A rabbit SAH model was established by injection of the autoblood via cisterna magna. Cerebral blood flow and arterial diameter were measured by Transcranial Doppler (TCD) and Computed Tomography Angiography (CTA). Expression of eNOS and PDE-V in basilar artery (BA) was assessed by western blots. Levels of nitric oxide (NO) in plasma and cerebral spinal fluid, and of intra-endothelium Ca2+ were measured. Significantly reduced diameter and accelerated blood flow velocity were detected in BAs of SAH animals (P