Protective effect of dietary azuki bean (Vigna angularis) seed coats against renal interstitial fibrosis of rats induced by cisplatin
Protective effect of dietary azuki bean (Vigna angularis) seed coats against renal interstitial fibrosis of rats induced by cisplatin
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DOI：
10.1016/j.nut.2004.07.019
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发表时间：
2005-04-01
期刊：
影响因子：
4.4
通讯作者：
Hatai, A
中科院分区：
文献类型：
--
作者：
Sato, S;Hori, Y;Hatai, A
目的：探讨以原花青素和膳食纤维为主成分的红豆种皮（ABSCs）对顺铂（CDDP）诱导的巨噬细胞浸润和肾间质纤维化进展的影响。方法：将雄性大鼠分为两组，对照组腹腔注射生理盐水，其余大鼠腹腔注射CDDP 3 mg/kg，每次1次。一周，持续 5 周。注射CDDP的动物接受四种饮食之一：1）对照饮食（仅商业饮食），2）0.5％红色ABSC（RABSC）饮食，3）2.0％RABSC饮食，和4）2.0％白色ABSC（WABSC）饮食。注射盐水的动物给予商业饮食。最后一次CDDP注射后五周，检查巨噬细胞动力学和间质纤维化面积。结果：RABSC中的多酚含量（76.3g/kg植物材料）高于WABSC中的多酚含量（18.1g/kg）。在 RABSC 中检测到原花青素 (20.4 g/kg)，但在 WABSC 中未检测到。组织学上，CDDP处理的大鼠肾脏中，由成纤维细胞和单核细胞组成的纤维化区域在皮质髓质交界处扩张或萎缩的小管周围形成，而WABSC和RABSC处理的大鼠中损伤的程度和程度较小。在免疫组织化学分析中，与对照组相比，CDDP治疗的大鼠中ED1阳性巨噬细胞的数量显着增加。 CDDP+WABSC或RABSC组的巨噬细胞数量明显少于CDDP治疗组的大鼠。此外，RABSC组中的巨噬细胞数量明显少于WABSC组，表明ABSC，特别是RABSC，阻止巨噬细胞浸润到间质纤维化区域。结论：这些结果表明ABSC，特别是RABSC，抑制受损肾脏中浸润巨噬细胞的增加，可能导致间质纤维化的改善。根据ABSC的组成，原花青素和/或膳食纤维等分子可能与肾损伤的改善有关。 (c) 2005 Elsevier Inc. 保留所有权利。
Objective: We investigated the effects of azuki bean (Vigna angularis) seed coats (ABSCs), which mainly contain proanthocyanidins and dietary fibers, on the infiltration of macrophages and the progression of renal interstitial fibrosis induced by cisplatin (CDDP).Methods: Male rats were divided into two groups: controls received intraperitoneal injections of saline and the other rats were injected intraperitoneally with 3 mg of CDDP per kilogram once a week for 5 wk. The CDDP-injected animals received one of four diets: 1) control diet (commercial diet only), 2) 0.5% red ABSC (RABSC) diet, 3) 2.0% RABSC diet, and 4) 2.0% white ABSC (WABSC) diet. The saline-injected animals were given the commercial diet. Five weeks after the final CDDP injection, macrophage kinetics and interstitial fibrotic areas were examined.Results: The content of polyphenols in the RABSC (76.3 g/kg of plant material) was higher than that in the WABSC (18.1 g/kg). Proanthocyanidins were detected in the RABSC (20.4 g/kg) but not in the WABSC. Histologically, the fibrotic areas consisting of fibroblastic cells and mononuclear cells developed around the dilated or atrophic tubules in the corticomedullary junction in CDDP-treated rat kidney, whereas the extent and magnitude of damage were less in the WABSC- and RABSC-treated rats. In immunohistochemical analysis, ED1-positive macrophages in CDDP-treated rats showed a significant increase in number compared with the control. The number of macrophages in CDDP plus WABSC or RABSC groups was significantly smaller than that in CDDP-treated rats. In addition, the number of macrophages in the RABSC group was significantly smaller than in the WABSC group, indicating that ABSC, especially RABSC, prevented macrophages from infiltrating into areas of interstitial fibrosis.Conclusions: These results suggest that ABSC, especially RABSC, suppress the increase of infiltrating macrophages in the damaged kidney and may lead to amelioration of interstitial fibrosis. Based on the composition of ABSC, molecules such as proanthocyanidins and/or dietary fibers may be associated with the amelioration of renal damage. (c) 2005 Elsevier Inc. All rights reserved.