The diversity of rab GTPases in Entamoeba histolytica
The diversity of rab GTPases in Entamoeba histolytica
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DOI：
10.1016/j.exppara.2005.02.021
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发表时间：
2005-07-01
影响因子：
2.1
通讯作者：
Nozaki, T
中科院分区：
文献类型：
--
作者：
Saito-Nakano, Y;Loftus, BJ;Nozaki, T
Rab蛋白是普遍存在的小gtp结合蛋白，形成一个高度保守的家族并调节囊泡运输。最近完成的肠原生动物寄生虫溶组织内阿米巴（Entamoeba histolytica）基因组使我们能够鉴定出极大量的假定Rab基因。对阿米巴、人类和酵母的Rab进行比对和系统发育分析，结果表明，阿米巴的22种Rab蛋白，包括EhRab1、EhRab2、EhRab5、EhRab7、EhRab8、EhRab11和EhRab21与其他生物的Rab具有显著的相似性。剩下的69个阿米巴Rab蛋白与其他生物的Rab蛋白只有适度的相似性（小于40%的相似性）。大约三分之一的rabb蛋白包括Rab7、Rab11和RabC，形成15个亚家族，包含多达9个亚型。大约70%的阿米巴rabb基因含有单个或多个内含子，这一比例明显高于该生物中普通基因的比例。25个Rabs具有非典型羧基末端，如CXXX， XCXX， XXCX， XXXC，没有半胱氨酸。我们提出了阿米巴Rab基因的注释，并讨论了这种生物体中ehrb蛋白的非凡多样性的生物学意义。(c) 2005爱思唯尔公司版权所有。
Rab proteins are ubiquitous small GTP-binding proteins that form a highly conserved family and regulate vesicular trafficking. Recent completion of the genome of the enteric protozoan parasite Entamoeba histolytica enabled us to identify an extremely large number (> 90) of putative Rab genes. Multiple alignment and phylogenic analysis of amebic, human, and yeast Rab showed that only 22 amebic Rab proteins including EhRab1, EhRab2, EhRab5, EhRab7, EhRab8, EhRab11, and EhRab21 showed significant similarity to Rab from other organisms. The 69 remaining amebic Rab proteins showed only moderate similarity (< 40% identity) to Rab proteins from other organisms. Approximately one-third of Rab proteins including Rab7, Rab11, and RabC form 15 subfamilies, which contain up to nine isoforms. Approximately 70% of amebic Rab genes contain single or multiple introns, and this proportion is significantly higher than that of common genes in this organism. Twenty-five Rabs possess an atypical carboxyl terminus such as CXXX, XCXX, XXCX, XXXC, and no cysteine. We propose annotation of amebic Rab genes and discuss biological significance of this extraordinary diversity of EhRab proteins in this organism. (c) 2005 Elsevier Inc. All rights reserved.