MEPE expression in osteocytes during orthodontic tooth movement
MEPE expression in osteocytes during orthodontic tooth movement
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DOI：
10.1016/j.archoralbio.2006.12.010
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发表时间：
2007-07-01
影响因子：
3
通讯作者：
Harris, S. E.
中科院分区：
文献类型：
--
作者：
Gluhak-Heinrich, J.;Pavlin, D.;Harris, S. E.
MEPE 和 DMPI 可能在骨细胞微环境中的矿化和脱矿中发挥作用。我们早期的研究表明 DMP1 具有机械响应性 [Gluhak-Heinrich J, Ye L, Bonewald LF, Feng JQ, MacDougall M, Harris SE, et al.机械负荷会刺激体内骨细胞中的牙本质基质蛋白 1 (DMP1)。骨敏研究杂志，2003； 18（5）：807-17]。目的：利用小鼠牙齿移动模型研究机械负荷对 MEPE 表达的影响，并将这种影响与 DMP1 的影响进行比较。方法：对加载 6-72 小时的 38 个处理骨位点和 38 个对照骨位点进行原位杂交和免疫组织化学分析。 ImageJ 用于骨细胞中 mRNA 表达的定量。结果：牙槽骨骨细胞表现出较高的 MEPE 基础水平，该水平在加载的第一天下降，随后在第 3 天刺激 2.8 倍，并在第 7 天恢复到对照水平。结论：与 DMP1 相比，MEPE 的骨细胞特异性机械刺激延迟且不同。这表明 MEPE 和 DMP1 在骨细胞对体内机械负荷的反应中发挥着独特的作用。 (c) 2006 Elsevier Ltd. 保留所有权利。
MEPE and DMPI may play a role in mineralisation and demineralisation within the osteocyte microenvironment. our earlier studies showed that DMP1 is mechanically responsive [Gluhak-Heinrich J, Ye L, Bonewald LF, Feng JQ, MacDougall M, Harris SE, et al. Mechanical loading stimulates dentin matrix protein 1 (DMP1) in osteocytes in vivo. J Bone Min Res 2003; 18(5):807-17]. Objectives: To examine the effect of mechanical loading on the expression of MEPE using mouse tooth movement model, and compare this effect to that on DMP1. Methods: In situ hybridisation and immunohistochemistry was performed on 38 treated and 38 control bone sites loaded 6-72 h. ImageJ was used for quantification of mRNA expression in osteocytes. Results: Alveolar osteocytes showed high basal level of MEPE that decreased during the first day of loading, followed by 2.8-fold stimulation at day 3, and returning to a control level by day 7. Conclusion: The osteocyte specific mechanical stimulation of MEPE was delayed and different, compared to that of DMP1. This suggests a distinct role of MEPE and DMP1 in the response of osteocytes to mechanical loading in vivo. (c) 2006 Elsevier Ltd. All rights reserved.