MOLECULAR-WEIGHTS OF LEPIDOPTERAN BACULOVIRUS DNAS - DERIVATION BY ELECTRON-MICROSCOPY
MOLECULAR-WEIGHTS OF LEPIDOPTERAN BACULOVIRUS DNAS - DERIVATION BY ELECTRON-MICROSCOPY
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DOI：
10.1099/0022-1317-37-3-501
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发表时间：
1977-01-01
影响因子：
3.8
通讯作者：
BURGESS, S
中科院分区：
文献类型：
--
作者：
BURGESS, S
从7个核型多角体病毒和3个颗粒体病毒中提取的共价闭合环状DNA的分子量估计值是通过比较它们的轮廓长度与f1噬菌体DNA复制形式的轮廓长度而得出的。对于所研究的10种病毒中的每一种，环状DNA分子的长度是均匀的。得到的MW值为58 × 104~58 × 104。106 - 94 ×106，但除2个值外，所有值都在69 × 106的范围内。106 - 81 ×106.在后一个范围内似乎有2个主要尺寸类别。3种颗粒体病毒DNA的分子量分别为68 × 10 - 6和68 × 10 - 6。106 - 71 ×106，而核型多角体病毒DNA跨越了整个范围。因此，从杆状病毒分离的共价闭合DNA中获得的MW值的分布与将该组划分为核型多角体病毒和颗粒体病毒不一致。
MW estimates were derived for the covalently-closed circular DNA extracted from 7 nuclear polyhedrosis and 3 granulosis viruses by a comparison of their contour length with that of the replicative form of f1 bacteriophage DNA. The length of the circular DNA molecules was uniform for each of the 10 viruses studied. The MW values obtained ranged from 58 .times. 106-94 .times. 106 although all but 2 values were within the range 69 .times. 106-81 .times. 106. There appeared to be 2 major size classes within the latter range. The MW of the 3 granulosis virus DNA were from 68 .times. 106-71 .times. 106 while the nuclear polyhedrosis virus DNA spanned the full range. Thus, the distribution of MW values obtained from covalently-closed DNA isolated from baculoviruses was not consistent with the division of the group into nuclear polyhedrosis and granulosis viruses.