Transporter-mediated biofuel secretion
Transporter-mediated biofuel secretion
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DOI：
10.1073/pnas.1301358110
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发表时间：
2013-05-07
影响因子：
11.1
通讯作者：
Chang, Geoffrey
中科院分区：
文献类型：
--
作者：
Doshi, Rupak;Tuan Nguyen;Chang, Geoffrey
利用微生物生产生物燃料是目前可再生能源领域最有前途的策略之一。然而，收获这些生物以提取生物燃料是能源和成本密集型的，限制了大规模生产的商业可行性。在这里，我们展示了一类具有药理意义的运输蛋白的使用，以避免在生物燃料生产过程中收获生物质的需要。我们发现，膜包埋的转运体，更为人所知的是外排脂类和药物，可以用来介导细胞内合成的模型异戊二烯生物燃料化合物分泌到细胞外环境。在我们的大肠杆菌测试系统中，转运蛋白介导的生物燃料分泌可持续地保持生物燃料产量的大约三到五倍的增长。由于本研究中使用的转运蛋白属于普遍存在的三磷酸腺苷结合盒蛋白家族，我们建议在用于生物燃料生产的各种细菌、蓝藻、硅藻、酵母和藻类中使用它们作为即插即用的生物燃料分泌系统。这项研究展示了在细胞工厂中表达所需的膜运输蛋白以实现具有经济、环境或治疗重要性的物质的出口或进口的潜力。
Engineering microorganisms to produce biofuels is currently among the most promising strategies in renewable energy. However, harvesting these organisms for extracting biofuels is energy-and cost-intensive, limiting the commercial feasibility of large-scale production. Here, we demonstrate the use of a class of transport proteins of pharmacological interest to circumvent the need to harvest biomass during biofuel production. We show that membrane-embedded transporters, better known to efflux lipids and drugs, can be used to mediate the secretion of intracellularly synthesized model isoprenoid biofuel compounds to the extracellular milieu. Transporter-mediated biofuel secretion sustainably maintained an approximate three-to fivefold boost in biofuel production in our Escherichia coli test system. Because the transporters used in this study belong to the ubiquitous ATP-binding cassette protein family, we propose their use as "plug-and-play" biofuel-secreting systems in a variety of bacteria, cyanobacteria, diatoms, yeast, and algae used for biofuel production. This investigation showcases the potential of expressing desired membrane transport proteins in cell factories to achieve the export or import of substances of economic, environmental, or therapeutic importance.