The cDNA Microarray analysis using an Arabidopsis pad3 mutant reveals the expression profiles and classification of genes induced by Alternaria brassicicola attack
The cDNA Microarray analysis using an Arabidopsis pad3 mutant reveals the expression profiles and classification of genes induced by Alternaria brassicicola attack
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DOI：
10.1093/pcp/pcg050
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发表时间：
2003-04-01
影响因子：
4.9
通讯作者：
Shinozaki, K
中科院分区：
文献类型：
--
作者：
Narusaka, Y;Narusaka, M;Shinozaki, K
通过接种芸苔链格孢菌，在野生型拟南芥植物（哥伦比亚）（Col-wt）中诱导过敏反应（HR），导致叶子上出现小的棕色坏死病斑。相比之下，用甘蓝曲霉攻击的pad3-1突变体产生了扩散性病变。尽管接种后 Col-wt 和 pad3-1 植物中 H2O2 的产生和局部细胞死亡相似，但 pad3-1 突变体中的细胞死亡不能抑制病原体的生长和发育。 Col-wt 和 pad3-1 植物之间的区别在于细胞死亡发生后的防御反应。换句话说，PAD3 对于对甘蓝菌的防御反应是必需的。因此，我们检查了 ca 表达模式的变化。接种 A. brasciicola 后，通过 cDNA 微阵列分析得出 7,000 个基因。 cDNA 微阵列还用于分析拟南芥在用信号分子、活性氧 (ROS) 诱导化合物和 UV-C 处理后的反应。结果表明，pad3-1 突变不仅改变了camalexin 的积累，还改变了许多防御相关基因响应甘蓝菌攻击的表达时间。此外，植物整合两个或多个共同作用的信号，以促进多种防御途径的诱导。
The hypersensitive response (HR) was induced in a wild-type Arabidopsis thaliana plant (Columbia) (Col-wt) by inoculation with Alternaria brassicicola that causes the development of small brown necrotic lesions on the leaves. By contrast, pad3-1 mutants challenged with A. brassicicola produced spreading lesions. The cell death in pad3-1 mutants could not inhibit the pathogen growth and development, although both production of H2O2 and localized cell death were similar in Col-wt and pad3-1 plants after the inoculation. The difference between Col-wt and pad3-1 plants is defense responses after the occurrence of cell death. In other words, PAD3 is necessary for defense response to A. brassicicola. Therefore, we examined the changes in the expression patterns of ca. 7,000 genes by cDNA microarray analysis after inoculation with A. brassicicola. The cDNA microarrays were also done to analyze Arabidopsis responses after treatment with signal molecules, reactive oxygen species (ROS)-inducing compounds and UV-C. The results suggested that the pad3-1 mutation altered not only the accumulation of camalexin but also the timing of expression of many defense-related genes in response to the challenge with A. brassicicola. Furthermore, the plants integrate two or more signals that act together for promoting the induction of multiple defense pathways.