Impact of Dietary Resistant Starch on the Human Gut Microbiome, Metaproteome, and Metabolome.
Impact of Dietary Resistant Starch on the Human Gut Microbiome, Metaproteome, and Metabolome.
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DOI：
10.1128/mbio.01343-17
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发表时间：
2017-10-17
期刊：
影响因子：
6.4
通讯作者：
Jansson JK
中科院分区：
文献类型：
--
作者：
Maier TV;Lucio M;Lee LH;VerBerkmoes NC;Brislawn CJ;Bernhardt J;Lamendella R;McDermott JE;Bergeron N;Heinzmann SS;Morton JT;González A;Ackermann G;Knight R;Riedel K;Krauss RM;Schmitt-Kopplin P;Jansson JK
饮食可以影响人类微生物组的组成，但相对较少的饮食成分被系统地调查了它们对微生物组功能潜力的影响。膳食抗性淀粉(RS)已被证明对健康有好处，但我们缺乏对RS消化过程中肠道代谢过程的机械性了解。在这里，我们在饮食交叉研究期间收集了样本，其中饮食中含有大量或少量的RS。我们采用了多种“组学”方法，包括16S rRNA基因测序、代谢蛋白质组学和代谢组学，确定了RS对肠道微生物组和肠道代谢途径的影响。这种多重组学方法捕捉到了高抗性淀粉(HRS)饮食后特定细菌物种、蛋白质和代谢物丰度的变化，为饮食干预对肠道微生物群的影响提供了关键的见解。综合数据表明，高RS饮食导致了Firmicmin与类杆菌的比率增加，包括Firmicmin某些特定成员的相对丰度增加，同时参与肠道脂肪代谢的酶途径和代谢物增加。这项工作是为了从机理上了解饮食和肠道中驻留的微生物之间的复杂相互作用。虽然已知肠道微生物在消化我们所消费的食物中起着关键作用，但不同微生物的具体贡献还不是很清楚。此外，消化过程中的代谢途径和代谢产物是非常复杂的。为了解决这些知识差距，我们使用了一系列分子方法来确定消化膳食淀粉过程中肠道微生物的身份以及它们进行的代谢途径。总而言之，这些数据提供了肠道微生物群在消化中的功能的更完整的图景，包括RS饮食和脂肪代谢之间的联系，以及特定肠道微生物与其代谢产物和肠道中产生的蛋白质之间的新联系。
Diet can influence the composition of the human microbiome, and yet relatively few dietary ingredients have been systematically investigated with respect to their impact on the functional potential of the microbiome. Dietary resistant starch (RS) has been shown to have health benefits, but we lack a mechanistic understanding of the metabolic processes that occur in the gut during digestion of RS. Here, we collected samples during a dietary crossover study with diets containing large or small amounts of RS. We determined the impact of RS on the gut microbiome and metabolic pathways in the gut, using a combination of “omics” approaches, including 16S rRNA gene sequencing, metaproteomics, and metabolomics. This multiomics approach captured changes in the abundance of specific bacterial species, proteins, and metabolites after a diet high in resistant starch (HRS), providing key insights into the influence of dietary interventions on the gut microbiome. The combined data showed that a high-RS diet caused an increase in the ratio of Firmicutes to Bacteroidetes, including increases in relative abundances of some specific members of the Firmicutes and concurrent increases in enzymatic pathways and metabolites involved in lipid metabolism in the gut. This work was undertaken to obtain a mechanistic understanding of the complex interplay between diet and the microorganisms residing in the intestine. Although it is known that gut microbes play a key role in digestion of the food that we consume, the specific contributions of different microorganisms are not well understood. In addition, the metabolic pathways and resultant products of metabolism during digestion are highly complex. To address these knowledge gaps, we used a combination of molecular approaches to determine the identities of the microorganisms in the gut during digestion of dietary starch as well as the metabolic pathways that they carry out. Together, these data provide a more complete picture of the function of the gut microbiome in digestion, including links between an RS diet and lipid metabolism and novel linkages between specific gut microbes and their metabolites and proteins produced in the gut.