High expression of Ran GTPase is associated with local invasion and metastasis of human clear cell renal cell carcinoma
High expression of Ran GTPase is associated with local invasion and metastasis of human clear cell renal cell carcinoma
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DOI：
10.1002/ijc.23400
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发表时间：
2008-05-15
影响因子：
6.4
通讯作者：
Yoshida, Ken-ichiro
中科院分区：
文献类型：
--
作者：
Abe, Hideyuki;Kamai, Takao;Yoshida, Ken-ichiro
Ran小GTdR（Ran）参与调节核运输、微管成核和动力学以及纺锤体组装。为了解决这个问题，是否Ran蛋白与肾细胞癌（RCC）的进展，我们比较了从180个连续的日本患者与相应的非肿瘤组织从同一患者的手术RCC标本中的Ran蛋白水平的Western印迹。我们还检测了不同级别和阶段的肿瘤中的Ran蛋白水平。Ran蛋白在肾细胞癌组织中的表达明显高于非癌组织（p < 0.0001）。Ran高表达与肿瘤的高分级、局部浸润和转移有关（P < 0.0001）。Kaplan-Meier曲线将Ran蛋白高表达与所有病例中较短的总生存期（p < 0.0001）和在根治性或部分肾切除术中无转移的患者中较短的无病生存期（MO; 131例，p < 0.0001）联系起来。Ran蛋白表达是影响总生存期的独立因素（单因素分析P < 0.0001），多因素分析P < 0.05）。我们的研究结果表明，Ran与RCC的进展有关。(C)2008 Wiley-Liss，Inc.
The Ran small GTPase (Ran) is involved in the regulation of nuclear transport, microtubule nucleation and dynamics, and spindle assembly. To address the question of whether Ran protein is associated with the progression of renal cell carcinoma (RCC), we compared by Western blotting the Ran protein levels in surgical RCC specimens from 180 consecutive Japanese patients with those in the corresponding nontumor tissue from the same patient. We also examined the Ran protein levels in tumors of different grades and stages. Ran proteins were more abundant in RCC tumor tissues than in nontumor tissues (p < 0.0001). High Ran expression was associated with higher grade, local invasion, and metastasis (P < 0.0001). Kaplan-Meier plots linked high Ran protein expression to a shorter overall survival in all cases (p < 0.0001) and a shorter disease-free survival in those without metastasis at radical or partial nephrectomy (MO; 131 cases, p < 0.0001). Ran protein expression was an independent factor influencing overall survival univariate analysis (p < 0.0001) and disease-free survival by multivariate analysis (p < 0.05). Our findings suggest that Ran is associated with the progression of RCC. (C) 2008 Wiley-Liss, Inc.