Alterations in the proteome of pulmonary alveolar type II cells in the rat after hepatic ischemia-reperfusion
Alterations in the proteome of pulmonary alveolar type II cells in the rat after hepatic ischemia-reperfusion
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DOI：
10.1097/ccm.0b013e31816f49cb
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发表时间：
2008-06-01
影响因子：
8.8
通讯作者：
Matthay, Michael A.
中科院分区：
文献类型：
--
作者：
Hirsch, Jan;Niemann, Claus U.;Matthay, Michael A.
目的：肝缺血再灌注可导致急性肺损伤。肺泡上皮II型细胞（ATII）在维持肺内环境稳定在急性肺injuries.Design的重要作用：研究潜在的新机制肝缺血-再灌注诱导的肺injury，我们研究了肝缺血-再灌注如何改变ATIISetting的蛋白质组：Laboratory investigation.Subjects：自主呼吸的雄性Zucker rats.Interventions：大鼠用异氟烷麻醉。阻断肝脏左叶和中叶的血管供应75分钟，然后再灌注。假手术大鼠作为对照。测量和主要结果：进行支气管肺泡灌洗和细胞分类计数，并通过酶联免疫吸附测定法测定血浆中肿瘤坏死因子-α和嘌呤诱导的中性粒细胞趋化因子-1。分离、裂解、胰蛋白酶消化ATII，并使用同量异序标签（iTRAQ）标记。样品经阳离子交换色谱分离，高效液相色谱分离，电喷雾串联质谱鉴定。使用ProteinProspector查询和定量光谱。定量蛋白质组学提供了两组中94和97种蛋白质的定量数据。ATII蛋白质含量的显著变化包括三磷酸腺苷酶、三磷酸腺苷/二磷酸腺苷转位酶和过氧化氢酶增加30%至40%（均为p
Objective: Hepatic ischemia-reperfusion can be associated with acute lung injury. Alveolar epithelial type II cells (ATII) play an important role in maintaining lung homeostasis in acute lung injury.Design: To study potentially new mechanisms of hepatic ischemia-reperfusion-induced lung injury, we examined how liver ischemia-reperfusion altered the proteome of ATIISetting: Laboratory investigation.Subjects: Spontaneously breathing male Zucker rats.Interventions: Rats were anesthetized with isoflurane. The vascular supply to the left and medial lobe of the liver was clamped for 75 mins and then reperfused. Sham-operated rats were used as controls. After 8 hrs, rats were killed.Measurements and Main Results: Bronchoalveolar lavage and differential cell counts were performed, and tumor necrosis factor-alpha and cytokine-induced neutrophil chemotactic factor-1 in plasma were determined by enzyme-linked immunosorbent assay. ATII were isolated, lysed, tryptically digested, and labeled using isobaric tags (iTRAQ). The samples were fractionated by cation exchange chromatography, separated by high-performance liquid-chromatography, and identified using electrospray tandem mass spectrometry. Spectra were interrogated and quantified using ProteinProspector. Quantitative proteomics provided quantitative data for 94 and 97 proteins in the two groups. Significant changes in ATII protein content included 30% to 40% increases in adenosine triphosphate synthases, adenosine triphosphate/adenosine diphosphate translocase, and catalase (all p