The role of nitric oxide during embryonic wound healing
The role of nitric oxide during embryonic wound healing
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DOI：
10.1186/s12864-019-6147-6
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发表时间：
2019-11-06
期刊：
影响因子：
4.4
通讯作者：
Sindelka, Radek
中科院分区：
文献类型：
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作者：
Abaffy, Pavel;Tomankova, Silvie;Sindelka, Radek
背景控制伤口愈合的机制的研究是生物学领域内的一个有吸引力的领域，鉴于目前与老年人群愈合相关的医疗负担，它对卫生部门具有潜在的重大影响。愈合是一个复杂的过程，包括许多由编码和非编码RNA，蛋白质和其他分子调节的步骤。一氧化氮（NO）是这些小分子调节剂之一，其功能已经与成人愈合过程中的炎症和血管生成有关。结果我们的研究结果表明，NO也是胚胎无瘢痕愈合过程中的重要组成部分，并通过以前未知的机制发挥作用。NO主要在愈合的早期阶段产生，它对与愈合相关的基因的表达至关重要。然而，我们还观察到愈合的晚期阶段，其发生在伤口闭合后数小时，发生在表皮下，包括依赖于NO的组织重塑。我们还发现NO与多种细胞代谢途径相关，特别是葡萄糖代谢途径。这是特别值得注意的，因为已经发现使用NO供体对于治疗慢性愈合缺陷（包括与糖尿病相关的那些）是有益的，并且其作用机制可能遵循在胚胎伤口愈合期间观察到的那些。结论：我们的研究描述了NO在愈合过程中的新作用，这可能会转化为改善治疗方法，特别是对有问题的愈合的个体痛苦。
Background The study of the mechanisms controlling wound healing is an attractive area within the field of biology, with it having a potentially significant impact on the health sector given the current medical burden associated with healing in the elderly population. Healing is a complex process and includes many steps that are regulated by coding and noncoding RNAs, proteins and other molecules. Nitric oxide (NO) is one of these small molecule regulators and its function has already been associated with inflammation and angiogenesis during adult healing. Results Our results showed that NO is also an essential component during embryonic scarless healing and acts via a previously unknown mechanism. NO is mainly produced during the early phase of healing and it is crucial for the expression of genes associated with healing. However, we also observed a late phase of healing, which occurs for several hours after wound closure and takes place under the epidermis and includes tissue remodelling that is dependent on NO. We also found that the NO is associated with multiple cellular metabolic pathways, in particularly the glucose metabolism pathway. This is particular noteworthy as the use of NO donors have already been found to be beneficial for the treatment of chronic healing defects (including those associated with diabetes) and it is possible that its mechanism of action follows those observed during embryonic wound healing. Conclusions Our study describes a new role of NO during healing, which may potentially translate to improved therapeutic treatments, especially for individual suffering with problematic healing.