A FAMILY OF 3 MOUSE POTASSIUM CHANNEL GENES WITH INTRONLESS CODING REGIONS
A FAMILY OF 3 MOUSE POTASSIUM CHANNEL GENES WITH INTRONLESS CODING REGIONS
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DOI：
10.1126/science.2305265
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发表时间：
1990-02-23
期刊：
影响因子：
56.9
通讯作者：
GUTMAN, GA
中科院分区：
文献类型：
--
作者：
CHANDY, KG;WILLIAMS, CB;GUTMAN, GA
为了理解负责在哺乳动物细胞中产生生理上不同的钾通道的分子机制，已经分离出具有钾通道互补DNA MBK1的小鼠基因组克隆，MBK1与果蝇钾通道基因Shaker同源。已经分离出在不同基因组位点编码的三个密切相关的钾通道基因（MK1、MK2和MK3）的家族。序列分析表明，这三个基因的编码区存在于小鼠基因组中作为一个单一的不间断的外显子。这种组织排除了通过选择性RNA剪接产生多种形式的蛋白质，这是一种已知的表征果蝇钾通道基因Shaker和shab的机制。因此，哺乳动物可能使用不同的策略来产生不同的K+通道，通过编码多个不同基因组位点的相关基因，每个基因组位点仅产生单一蛋白质。
To understand the molecular mechanisms responsible for generating physiologically diverse potassium channels in mammalian cells, mouse genomic clones have been isolated with a potassium channel complementary DNA, MBK1, that is homologous to the Drosophila potassium channel gene, Shaker. A family of three closely related potassium channel genes (MK1, MK2, and MK3) that are encoded at distinct genomic loci has been isolated. Sequence analysis reveals that the coding region of each of these three genes exists as a single uninterrupted exon in the mouse genome. This organization precludes the generation of multiple forms of the protein by alternative RNA splicing, a mechanism known to characterize the Drosophila potassium channel genes Shaker and shab. Thus, mammals may use a different stategy for generating diverse K+ channels by encoding related genes at multiple distinct genomic loci, each of which produces only a single protein.