Distribution of NADPH-diaphorase-positive neurons in the prefrontal cortex of the Cebus monkey
Distribution of NADPH-diaphorase-positive neurons in the prefrontal cortex of the Cebus monkey
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DOI：
10.1016/j.brainres.2006.01.098
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发表时间：
2006-04-14
期刊：
影响因子：
2.9
通讯作者：
Casatti, CA
中科院分区：
文献类型：
--
作者：
Cruz-Rizzolo, RJ;Horta-Júnior, JAC;Casatti, CA
应用NADPH黄递酶（NADPH-d）组织化学方法，研究了正常成年无尾猴前额叶皮层中NADPH-d活性的分布。研究了以下区域：颗粒区46和12，颗粒不良区9和13，无颗粒区32和Oap。NADPH-d阳性神经元分为两种不同类型，均为非锥体细胞。I型神经元具有大的索马直径（17.24 +/- 1.73 pm）并且被密集染色。这些神经元中90%以上位于皮质下白色质和颗粒下层。其余的I型神经元分布在颗粒上层。Ⅱ型神经元有一个小的，圆形或椭圆形的索马（9.83 +/- 1.03 μ m），其染色模式变化明显。II型神经元分布在整个皮质，其最大的数密度被观察到在第二和第三层。在颗粒区，II型神经元的数量是I型神经元的20倍，但这一比例在无颗粒区较小。Ⅱ型神经元的面密度在颗粒区的颗粒上层最大，在无颗粒区最小。统计分析表明，这些地区的差异是显着的，当比较一些特定的地区。总之，我们的研究结果表明，NADPH-d阳性细胞的优势颗粒层的颗粒区在Cebus前额叶皮质。这些研究结果支持先前的观察II型神经元作为一个新的皮质一氧化氮源在灵长类动物的颗粒上皮层层的作用，以及其在高级哺乳动物的皮质神经元激活的潜在贡献。(c)2006 Elsevier B. V.保留所有权利。
We studied the distribution of NADPH-diaphorase (NADPH-d) activity in the prefrontal cortex of normal adult Cebus apella monkeys using NADPH-d histochemical protocols. The following regions were studied: granular areas 46 and 12, dysgranular areas 9 and 13, and agranular areas 32 and Oap. NADPH-d-positive neurons were divided into two distinct types, both non-pyramidal. Type I neurons had a large soma diameter (17.24 +/- 1.73 pm) and were densely stained. More than 90% of these neurons were located in the subcortical white matter and infragranular layers. The remaining type I neurons were distributed in the supragranular layers. Type II neurons had a small, round or oval soma (9.83 +/- 1.03 mu m), and their staining pattern varied markedly. Type II neurons were distributed throughout the cortex, with their greatest numerical density being observed in layers II and III. In granular areas, the number of type II neurons was up to 20 times that of type I neurons, but this proportion was smaller in agranular areas. Areal density of type II neurons was maximum in the supragranular layers of granular areas and minimum in agranular areas. Statistical analysis revealed that these areal differences were significant when comparing some specific areas. In conclusion, our results indicate a predominance of NADPH-d-positive cells in supragranular layers of granular areas in the Cebus prefrontal cortex. These findings support previous observations on the role of type II neurons as a new cortical nitric oxide source in supragranular cortical layers in primates, and their potential contribution to cortical neuronal activation in advanced mammals. (c) 2006 Elsevier B.V. All rights reserved.