Functional activation of a novel R2R3-MYB protein gene, GmMYB68, confers salt-alkali resistance in soybean (Glycine max L.)
Functional activation of a novel R2R3-MYB protein gene, GmMYB68, confers salt-alkali resistance in soybean (Glycine max L.)
复制标题
新型 R2R3-MYB 蛋白基因 GmMYB68 的功能激活赋予大豆 (Glycine max L.) 耐盐碱能力。
DOI：
10.1139/gen-2018-0132
复制
发表时间：
2020-01-01
期刊：
影响因子：
3.1
通讯作者：
Wang, Qingyu
中科院分区：
文献类型：
--
作者：
He, Yuxuan;Dong, Yingshan;Wang, Qingyu
土壤盐分显著降低大豆（Glycine max L.）全球生产。植物对逆境胁迫的抗性是一个受多种信号通路调控的复杂特性。v-Myb禽成髓细胞瘤病毒癌基因同源物（MYB）转录因子（TF）在植物发育、次生代谢和非生物胁迫响应中起着至关重要的作用。建立了GmMYB 68过表达和RNA干扰（RNAi）细胞系，以检测GmMYB 68的功能。max GmMYB 68在植物对非生物胁迫响应中的作用。GmMYB 68的氨基酸序列与R2 R3-MYB蛋白的氨基酸序列相似。实时荧光定量PCR分析表明，GmMYB 68的表达变化响应非生物胁迫。GmMYB 68基因过表达株系对盐碱胁迫的抗性增强，其渗透调节能力和光合速率也强于GmMYB 68-RNAi和野生型。虽然野生型和转基因植株在正常条件下的农艺性状没有显着差异，但GmMYB 68的过表达增加了盐胁迫下的粒数和百粒重。我们的研究发现了一个与大豆胁迫反应相关的有价值的TF，因为它的过表达可能有助于提高大豆和其他作物的耐盐性和耐碱性。
Soil salinity significantly reduces soybean (Glycine max L.) production worldwide. Plants resistance to stress conditions is a complex characteristic regulated by multiple signaling pathways. The v-Myb avian myeloblastosis viral oncogene homolog (MYB) transcription factor (TF) plays a crucial role in plant development, secondary metabolism, and abiotic stress responses. GmMYB68-overexpression and RNA interference (RNAi) lines were established for examining the function of G. max GmMYB68 in plant responses to abiotic stresses. The predicted amino acid sequence of GmMYB68 was similar to that of R2R3-MYB proteins. Quantitative real-time PCR analysis revealed that GmMYB68 expression varied in response to abiotic stresses. GmMYB68-overexpression lines showed enhanced resistance to salt and alkali stresses and their osmotic adjustment and photosynthetic rates were also stronger than that of GmMYB68-RNAi and wild type plants. Although wild type and transgenic plants showed no significant differences in agronomic traits under normal conditions, the overexpression of GmMYB68 increased grain number and 100-grain weights under salt stress. Our study identified a valuable TF associated with stress response in soybean, as its overexpression might help improve salt and alkali tolerance in soybean and other crops.