Sickness behavior of rats with abdominal sepsis can be improved by antibiotic and G-CSF prophylaxis in clinic modeling randomized trials
Sickness behavior of rats with abdominal sepsis can be improved by antibiotic and G-CSF prophylaxis in clinic modeling randomized trials
复制标题
临床随机模型试验中抗生素和 G-CSF 预防可改善腹部脓毒症大鼠的疾病行为
作者：
A. Bauhofer;R. Schwarting;M. Köster;A. Schmitt;W. Lorenz;C. Pawlak
背景与目的：在脓毒症的临床研究中，几乎所有的免疫调节剂都没有显示出对28天死亡率有任何益处。其他终点，如生活质量，已经变得更具吸引力，但临床相关的动物模型通过测量疾病行为来分析等同于生活质量的动物模型是极其罕见的。方法：成年雄性Wistar大鼠80只，随机分为(1)对照组：麻醉和假手术；(2)败血症：剖腹手术和腹膜感染人类粪便细菌；(3)败血症+抗生素预防：头孢呋辛/甲硝唑；(4)败血症+抗生素+粒细胞集落刺激因子(GCSF)预防。终点是生理和行为参数。结果：抗生素联合G-CSF对降低死亡率效果最好。所有感染的动物在感染后都表现出明显的野外活动减少，并在预防治疗的9天内改善了恢复。在社会交往试验中，G-CSF与抗生素联合应用可有效改善脓毒症大鼠的患病行为，但在高架+迷宫焦虑试验中效果不明显。
Abstract.Background and aim: In clinical sepsis research nearly all immune-modulators have demonstrated no benefit in regard to the 28-day mortality rate. Other endpoints such as quality of life have become more attractive, but clinically relevant animal models analyzing an equivalent to quality of life by measurement of sickness behavior are extremely rare. The concept of clinic modeling randomized trials was used in an animal trial to model clinical complexity and conditions of a randomized clinical trial.Methods: 80 adult male Wistar rats were randomly assigned to (1) control: anesthesia and sham operation, (2) sepsis: laparotomy and peritoneal infection with human stool bacteria, (3) sepsis with antibiotic prophylaxis: cefuroxime/metronidazole and (4) sepsis with antibiotic plus a cytokine prophylaxis with granulocyte-colony stimulating factor (GCSF). Endpoints were physiological and behavioral parameters.Results: The combination of antibiotics plus G-CSF was most effective in reducing mortality. All infected animals showed reduced open field activity acutely after infection, and recovery was improved during the 9 day follow-up in rats with prophylactic treatments. In the social interaction test, but not in the elevated plus-maze anxiety test, prophylaxis was also efficient, especially with antibiotics and G-CSF.Conclusions: The results show that improving sickness behavior in septic rats with G-CSF plus antibiotics may be a promising approach.
DOI：
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发表时间：
1992
期刊：
Laboratory animal science
影响因子：
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作者：
Bradfield,JF;Schachtman,TR;McLaughlin,RM;Steffen,EK
通讯作者：
Steffen,EK