Regional differences in brain-derived neurotrophic factor levels and dendritic spine density confer resilience to inescapable stress.
Regional differences in brain-derived neurotrophic factor levels and dendritic spine density confer resilience to inescapable stress.
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DOI：
10.1093/ijnp/pyu121
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发表时间：
2015-01-07
期刊：
影响因子：
--
通讯作者：
Hashimoto K
中科院分区：
文献类型：
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作者：
Yang C;Shirayama Y;Zhang JC;Ren Q;Hashimoto K
在习得性无助 (LH) 范式中，大约 35% 的老鼠能够抵御不可避免的压力。研究了脑源性神经营养因子 (BDNF) 和树突棘密度在 LH（易感）和非 LH 大鼠（弹性）大脑区域中的作用。进行蛋白质印迹分析和高尔基体染色。 LH组内侧前额叶皮层、CA3和齿状回（DG）中的BDNF水平显着低于对照组和非LH组，而LH组伏核（NAc）中的BDNF水平显着高于对照组，而非LH组则没有。此外，LH 组的前边缘皮质、CA3 和 DG 中的棘密度显着低于对照组和非 LH 组，尽管 LH 组的 NAc 中的棘密度显着高于对照组和非 LH 组。结果表明，大鼠大脑中 BDNF 水平和脊柱密度的区域差异可能有助于增强对不可避免的压力的恢复能力。
In the learned helplessness (LH) paradigm, approximately 35% of rats are resilient to inescapable stress. The roles of brain-derived neurotrophic factor (BDNF) and dendritic spine density in the brain regions of LH (susceptible) and non-LH rats (resilient) were examined. Western blot analysis and Golgi staining were performed. BDNF levels in the medial prefrontal cortex, CA3, and dentate gyrus (DG) were significantly lower in the LH group than in the control and non-LH groups, whereas BDNF levels in the nucleus accumbens (NAc) in the LH group but not the non-LH group were significantly higher than those in the control group. Furthermore, spine density in the prelimbic cortex, CA3, and DG was significantly lower in the LH group than in the control and non-LH groups, although spine density in the NAc was significantly higher in the LH group than in the control and non-LH groups. The results suggest that regional differences in BDNF levels and spine density in rat brain may contribute to resilience to inescapable stress.