Using europium(III) complex of 1,4,7,10-tetraazacyclododecane-1,4,7-triacedic acid Eu.DO3A as a luminescent sensor for bicarbonate
Using europium(III) complex of 1,4,7,10-tetraazacyclododecane-1,4,7-triacedic acid Eu.DO3A as a luminescent sensor for bicarbonate
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DOI：
10.1016/j.jre.2019.11.017
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发表时间：
2020-05-01
影响因子：
4.9
通讯作者：
Sorensen, Thomas Just
中科院分区：
文献类型：
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作者：
Bridou, Lucile;Nielsen, Lea Gundorff;Sorensen, Thomas Just
检测生物体液和生物技术生产介质中的分析物仍然是一项挑战。本文详细研究了1，4，7，10-tetraazacyclododecane-1，4，7-triacetic酸Eu(Ⅲ)配合物(DO3A)对碳酸氢盐的发光响应。研究了不同缓冲液中重碳酸盐浓度变化对Eu DO3A的响应，确定了离子强度、pH和特定离子的影响。研究发现，响应依赖于pH和离子强度，但不可能将传感事件的贡献与碳酸氢盐活性的贡献分开。在演示确定生物生产介质和血清中重碳酸盐浓度的能力之前，先评估竞争羧基阴离子的影响。已经确定，虽然竞争阴离子与响应性络合物结合得更强，但在相关浓度下，它们对记录信号的贡献可以忽略不计。因此，我们能够得出结论，EU.DO3A是构建碳酸氢盐传感器的良好平台。(三)2020年中国稀土学会。爱思唯尔出版，版权所有。
Sensing of analytes in biological fluids and biotechnological production media remains a challenge. Here, the luminescent response of the europium (III) complex of 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (DO3A) to bicarbonate was investigated in detail. The response of Eu.DO3A to changes in bicarbonate concentration was studied in different buffers, and the influence of ionic strength, pH, and specific ions was determined. The response is found to depend on pH and ionic strength, but it is not possible to separate contributions from the sensing event from those in the bicarbonate activity. Prior to demonstrating the ability to determine the bicarbonate concentration in bioproduction media and serum, the influence of competing carboxy anions was evaluated. It is established that while competing anions bind stronger to the responsive complex, at the relevant concentration their contribution to the recorded signal is negligible. We are thus able to conclude that Eu.DO3A is a good platform for building a bicarbonate sensor. (C) 2020 Chinese Society of Rare Earths. Published by Elsevier B.V. All rights reserved.