Comparative Evaluation of Hepatoprotective Activities of Geniposide, Crocins and Crocetin by CCl4-Induced liver Injury in Mice.
Comparative Evaluation of Hepatoprotective Activities of Geniposide, Crocins and Crocetin by CCl4-Induced liver Injury in Mice.
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京尼平苷、藏红花素和藏红花酸对 CCl4 诱导的小鼠肝损伤的保肝活性的比较评价。
DOI：
10.4062/biomolther.2015.094
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发表时间：
2016-03-01
影响因子：
3.7
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Chen P;Chen Y;Wang Y;Cai S;Deng L;Liu J;Zhang H
环烯醚萜苷（主要是京尼平苷）和西红花酸衍生物（西红花苷）是栀子中的两种主要活性成分。本研究从栀子中分离得到栀子苷、西红花苷、西红花苷-1和西红花酸。然后，小鼠口服给予京尼平苷（400 mg/kg b.w.），西红花苷（400 mg/kg b.w.），藏红花素-1（400 mg/kg b.w.）和西红花酸（140 mg/kg体重）每日一次，连续7天。通过生化指标评价其肝保护特性：京尼平苷、西红花苷、西红花苷-1和西红花酸显著降低CCl 4处理小鼠血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）和碱性磷酸酶（ALP）水平。京尼平苷、西红花苷、西红花苷-1和西红花酸均能提高小鼠体内还原型谷胱甘肽（GSH）水平和抗氧化酶（SOD和CAT）活性。肝脏组织学检查表明，这些成分降低了CCl_4处理小鼠肝细胞的变形性，使肝细胞排列不规则，并使肝细胞破裂。这些生化结果和肝脏组织病理学评估表明，京尼平苷，藏红花酸衍生物和藏红花酸显示出比较有益的影响，四氯化碳诱导的肝损伤，通过诱导抗氧化防御。因此，栀子中栀子苷和西红花酸衍生物的含量应同时考虑其保肝作用。
Iridoid glycosides (mainly geniposide) and crocetin derivatives (crocins) are the two major active constituents in Gardenia jasminoides Ellis. In the present study, geniposide, crocins, crocin-1 and crocetin were separated from gardenia chromatographically. Then, mice were orally administrated with geniposide (400 mg/kg b.w.), crocins (400 mg/kg b.w.), crocin-1 (400 mg/kg b.w.) and crocetin (140 mg/kg b.w.) once daily for 7 days with CCl4. Hepatoprotective properties were evaluated by biochemical parameters: Administration of geniposide, crocins, crocin-1and crocetin significantly lowered serum alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) levels in CCl4-treated mice. The reduced glutathione (GSH) levels and antioxidant enzymes (SOD and CAT) activities were also increased by geniposide, crocins, crocin-1 and crocetin. Histopathological examination of livers showed that these components reduced deformability, irregular arrangement and rupture of hepatocyte in CCl4-treated mice. These biochemical results and liver histopathological assessment demonstrated that geniposide, crocetin derivatives and crocetin show comparative beneficial effects on CCl4-induced liver damage via induction of antioxidant defense. Therefore, contents of geniposide and crocetin derivatives should be both considered for hepatoprotective efficacy of Gardenia jasminoides Ellis.