Respiratory Syncytial Virus-A ON1 Genotype Emergence in Central Mexico in 2009 and Evidence of Multiple Duplication Events
Respiratory Syncytial Virus-A ON1 Genotype Emergence in Central Mexico in 2009 and Evidence of Multiple Duplication Events
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DOI：
10.1093/infdis/jiy025
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发表时间：
2018-04-01
影响因子：
6.4
通讯作者：
Bernal-Silva, Sofia
中科院分区：
文献类型：
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作者：
Comas-Garcia, Andreu;Noyola, Daniel E.;Bernal-Silva, Sofia
背景。呼吸道合胞病毒（RSV）是呼吸道感染的主要原因。 2012 年报道了包含 72 nt 重复的 RSV-A 基因型 (ON1)，此后已在全球范围内推广。我们分析了 2003 年至 2014 年间获得的 345 份呼吸道样本，以评估 ON1 感染的相关性。对在圣路易斯波托西检测到的病毒的核苷酸序列和推导的氨基酸序列以及之前报告的序列进行了分析。结果。在 105 个样本中检测到 RSV ON1。最早的 ON1 感染病例于 2009 年 11 月发现，比加拿大发现该病毒早了近一年。重复区的氨基酸序列分析表明，RSV GA2 病毒中存在 Y273N 和 L274P 取代，当组合时，在位置 272-278 产生 4 个不同的 GXXSPSQ 序列基序。其中三个基序同时存在于 ON1 菌株的原始区域和复制区域中。在具有不同序列基序的 ON1 菌株中观察到额外的特征氨基酸取代。结论。 ON1 毒株包含的病毒似乎是至少 3 个独立复制事件的结果。来自不同地理区域的菌株的分子数据应有助于确定这种进化机制的频率和影响。
Background. Respiratory syncytial virus (RSV) is a leading cause of respiratory infections. An RSV-A genotype (ON1) that contains a 72-nt duplication was reported in 2012 and has since extended worldwide.Methods. We analyzed 345 respiratory samples obtained between 2003 and 2014 to assess the relevance of ON1 infections. Nucleotidic and deduced amino acid sequences from viruses detected in San Luis Potosi and sequences previously reported were analyzed.Results. RSV ON1 was detected in 105 samples. The earliest case of ON1 infection was detected in November 2009, almost 1 year prior to detection of this virus in Canada. Amino acid sequence analysis of the duplication region showed the presence of Y273N and L274P substitutions in RSV GA2 viruses that, when combined, resulted in 4 different GXXSPSQ sequence motifs at positions 272-278. Three of these motifs were present in both the original and duplicated regions of ON1 strains. Additional signature amino acid substitutions were observed in ON1 strains that have the different sequence motifs.Conclusions. ON1 strains include viruses that appear to be the result of at least 3 independent duplication events. Molecular data of strains from diverse geographical regions should help define the frequency and implications of this evolution mechanism.