Sensitive and selective turn-off-on fluorescence detection of Hg 2+ and cysteine using nitrogen doped carbon nanodots derived from citron and urine
Sensitive and selective turn-off-on fluorescence detection of Hg 2+ and cysteine using nitrogen doped carbon nanodots derived from citron and urine
复制标题
DOI：
10.1016/j.snb.2017.12.046
复制
发表时间：
2017-12
影响因子：
8.4
通讯作者：
S. Stanly John Xavier;G. Siva;J. Annaraj;A. Kim;Dong Jin Yoo;G. Gnana kumar
中科院分区：
文献类型：
--
作者：
S. Stanly John Xavier;G. Siva;J. Annaraj;A. Kim;Dong Jin Yoo;G. Gnana kumar
以香橼果实提取物和人尿液为碳源和氮源制备氮掺杂碳纳米点（NCND），构建了一种环境友好、成本低廉的半胱氨酸荧光传感器。利用Hg 2+通过电子转移过程猝灭NCND的初始荧光，建立了半胱氨酸的关-开荧光传感技术。由于Hg 2+与半胱氨酸的竞争性结合，“关闭”荧光传感器被切换到“打开”状态。在优化的条件下，NCNDs-Hg 2+组装显示出较低的检测限和宽的半胱氨酸检测线性范围。此外，NCND显示半胱氨酸与相似生物活性分子的区别性检测，并且干扰物质的共存不影响NCND对分析物的检测。同时，通过对人体尿液样品的检测，验证了该传感器的实际应用价值，为生物分子的荧光检测开辟了新的途径。
An environmentally benign and cost efficient cysteine fluorescence sensor is constructed on the basis of nitrogen doped carbon nanodots (NCNDs) prepared by using citron fruit extract and human urine waste as carbon and nitrogen sources, respectively. The turn-off-on fluorescence technique is exploited for the sensing of cysteine, in which the initial fluorescence of NCNDs is quenched with Hg2+viaan electron transfer process. Owing to the competitive binding of Hg2+with cysteine, the ‘turn-off’ fluorescence sensor is switched to the ‘turn-on’ state. Under optimized conditions, NCNDs-Hg2+assembly demonstrates a lower detection limit and a wide linear range toward cysteine detection. Furthermore, NCNDs display the discriminative detection of cysteine from the similar bioactive molecules and the co-existence of interference species does not influence the detection of NCNDs toward the interest of an analyte. Furthermore, the practical application of NCNDs based fluorescence sensor is validated with the good recovery in human urine samples, which explores the new avenues in the fluorescence sensing of biomolecules.