Local and global changes in cell density induce reorganisation of 3D packing in a proliferating epithelium.
Local and global changes in cell density induce reorganisation of 3D packing in a proliferating epithelium.
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细胞密度的局部和整体变化诱导增殖上皮中 3D 堆积的重组。
DOI：
10.1101/2024.02.08.579268
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Escudero,LuisM
中科院分区：
文献类型：
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作者：
Barone,Vanessa;Tagua,Antonio;Andrés-SanRomán,JesusÁ;Hamdoun,Amro;Garrido-García,Juan;Lyons,DeirdreC;Escudero,LuisM
组织形态发生与单个细胞的形状和组织的变化密切相关。在弯曲的上皮细胞中，细胞可以沿着它们自己的根尖基轴插入，采用一种名为“盾形”的形状，使组织中的能量最小化。尽管一些几何和生物物理因素与这种3D重组有关，但3D上皮填塞中盾形形成的动态变化仍然知之甚少。在这里，我们使用海星胚胎的实时成像结合基于深度学习的分割来分析细胞密度、组织致密化和细胞增殖对上皮结构的相对贡献。我们发现，在胚胎中自然发生的组织紧致对于盾片的出现是必要的。物理压缩实验表明，细胞密度是在全球范围内促进盾形形成的因素。最后，对发育中的胚胎与计算模型的比较表明，盾片比例的增加与细胞分裂直接相关。我们的结果表明，在有丝分裂后立即出现的顶端-底端嵌合可能有助于适应组织内的新细胞。我们认为，致密上皮中的增殖诱导发育过程中的3D细胞重排。
Tissue morphogenesis is intimately linked to the changes in shape and organisation of individual cells. In curved epithelia, cells can intercalate along their own apicobasal axes, adopting a shape named ‘scutoid’ that allows energy minimization in the tissue. Although several geometric and biophysical factors have been associated with this 3D reorganisation, the dynamic changes underlying scutoid formation in 3D epithelial packing remain poorly understood. Here, we use live imaging of the sea star embryo coupled with deep learning-based segmentation to dissect the relative contributions of cell density, tissue compaction and cell proliferation on epithelial architecture. We find that tissue compaction, which naturally occurs in the embryo, is necessary for the appearance of scutoids. Physical compression experiments identify cell density as the factor promoting scutoid formation at a global level. Finally, the comparison of the developing embryo with computational models indicates that the increase in the proportion of scutoids is directly associated with cell divisions. Our results suggest that apico-basal intercalations appearing immediately after mitosis may help accommodate the new cells within the tissue. We propose that proliferation in a compact epithelium induces 3D cell rearrangements during development.