Model of selective gene therapy of hair growth: liposome targeting of the active Lac-Z gene to hair follicles of histocultured skin.
Model of selective gene therapy of hair growth: liposome targeting of the active Lac-Z gene to hair follicles of histocultured skin.
复制标题
毛发生长的选择性基因治疗模型：活性 Lac-Z 基因的脂质体靶向组织培养皮肤的毛囊。
DOI：
10.1007/bf02631332
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Hoffman,RM
中科院分区：
文献类型：
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作者：
Li,L;Hoffman,RM
尊敬的编辑：在海绵凝胶基质上的完整皮肤的组织培养为组织工程和皮肤固有的重要过程如毛发生长和对各种试剂的毒性的体外研究提供了许多机会（Li等人，1991年、1992年a、1992年b、1992年c）。我们最近利用该系统证明脂质体可以选择性地靶向毛囊递送包括DNA在内的小分子和大分子，脂质体已被广泛地成功地用作递送系统，将不能正常穿过质膜的大分子物质转运到细胞中（Gregoriadis 1984，1988）。我们最近报道了钙黄绿素染料包埋的脂质体特异性靶向组织培养的完整皮肤中的毛囊（Li等人，1992年d）。我们的研究结果得到了Lieb等人的有力证实。（1992），证明脂质体制剂比任何其它测试的载体更有效地通过毛囊途径用仓鼠耳模型将羧基荧光素递送到毛囊单位中。然后，我们开发了脂质体介导的黑色素靶向递送到组织培养的白发小鼠皮肤中的毛囊和毛干本身中（Li等人，1993年a）。最近，Yarosh还报道了脂质体允许T4内切核酸酶V渗透到活皮肤中（Yarosh et al.，1992年）。这些研究有许多分支，包括有针对性的头发生长的修改。
Dear Editor: The histoculture of intact skin on sponge-gel matrices offers many opportunities for tissue engineering and the in vitro study of important processes intrinsic to skin such as hair growth and toxicity to various agents (Li et al., 1991, 1992a, 1992b, 1992c). We have recently exploited this system to demonstrate that liposomes can selectively target hair follicles to deliver small and large molecules including DNA.Liposomes have been widely and successfully used as delivery systems to transport macromolecular substances that can not nor-mally cross the plasma membrane into the cell (Gregoriadis 1984, 1988). We have recently reported that calcein-dye entrapped liposomes specifically targeted hair follicles in histocultured intact skin (Li et al., 1992d). Our results were strongly confirmed by studies by Lieb et al.(1992) demonstrating that liposomal formulations were more efficient than any other vehicle tested for delivery of carboxyfluorescein through the follicular route into the pilose-baceous units with the hamster ear model. We then developed liposome-mediated targeted delivery of melanin into hair follicles and the hair shaft itself in histocultured white-haired mouse skin (Li et al., 1993a). Recently, Yarosh also reported that liposomes allow T4 endonuclease V to penetrate into living skin (Yarosh et al., 1992). These studies have many ramifications including targeted hair growth modifications.