Hybrid magnetite nanoparticles/Rosmarinus officinalis essential oil nanobiosystem with antibiofilm activity.
Hybrid magnetite nanoparticles/Rosmarinus officinalis essential oil nanobiosystem with antibiofilm activity.
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DOI：
10.1186/1556-276x-7-209
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发表时间：
2012-04-10
影响因子：
--
通讯作者：
Andronescu E
中科院分区：
文献类型：
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作者：
Chifiriuc C;Grumezescu V;Grumezescu AM;Saviuc C;Lazăr V;Andronescu E
由真菌生物体形成的生物膜与对大多数抗微生物剂的显著增强的抗性相关，尽管进行了抗真菌治疗，但仍有助于真菌的持久性。本研究的目的是将纳米颗粒的独特性质与迷迭香精油的抗微生物活性联合收割机，以获得可以在导管件表面上成膜的纳米生物系统，以获得对白色念珠菌和C.热带假丝酵母临床菌株中华民国采用Neo-Clevenger型萃取装置提取中药挥发油，用GC-MS分析其化学成分。以油酸为表面活性剂，采用微波沉淀法合成了粒径为20 nm的功能化纳米磁铁矿颗粒。通过在纳米流体上施加磁场，用悬浮的核/壳纳米颗粒（Fe 3 O 4/油酸：CHCl 3）涂覆导管件，同时通过在二次覆盖处理中吸附施加CHCl 3稀释的精油。真菌的粘附能力进行了研究，在六个多孔板，其中已放置导管件与和没有混合纳米粒子/精油纳米生物系统薄膜，通过使用基于培养的方法和共聚焦激光扫描显微镜（CLSM）。中华民国药用植物精油包裹的纳米粒强烈抑制了C.白色念珠菌和C. Tropicalis试验菌株粘附于导管表面，如活细胞计数和CLSM检查所示。由于念珠菌属的重要意义。在人类发病机制中，特别是在假体装置相关感染和抗真菌耐受性/耐药性的出现中，使用基于R.抑制真菌粘附的药用植物可能是生物医学领域的极大兴趣，为具有抗真菌膜特性的膜包衣表面的设计开辟了新的方向。
Biofilms formed by fungal organisms are associated with drastically enhanced resistance against most antimicrobial agents, contributing to the persistence of the fungi despite antifungal therapy. The purpose of this study is to combine the unique properties of nanoparticles with the antimicrobial activity of the Rosmarinus officinalis essential oil in order to obtain a nanobiosystem that could be pelliculised on the surface of catheter pieces, in order to obtain an improved resistance to microbial colonization and biofilm development by Candida albicans and C. tropicalis clinical strains. The R. officinalis essential oils were extracted in a Neo-Clevenger type apparatus, and its chemical composition was settled by GC-MS analysis. Functionalized magnetite nanoparticles of up to 20 nm size had been synthesized by precipitation method adapted for microwave conditions, with oleic acid as surfactant. The catheter pieces were coated with suspended core/shell nanoparticles (Fe3O4/oleic acid:CHCl3), by applying a magnetic field on nanofluid, while the CHCl3 diluted essential oil was applied by adsorption in a secondary covering treatment. The fungal adherence ability was investigated in six multiwell plates, in which there have been placed catheters pieces with and without hybrid nanoparticles/essential oil nanobiosystem pellicle, by using culture-based methods and confocal laser scanning microscopy (CLSM). The R. officinalis essential oil coated nanoparticles strongly inhibited the adherence ability and biofilm development of the C. albicans and C. tropicalis tested strains to the catheter surface, as shown by viable cell counts and CLSM examination. Due to the important implications of Candida spp. in human pathogenesis, especially in prosthetic devices related infections and the emergence of antifungal tolerance/resistance, using the new core/shell/coated shell based on essential oil of R. officinalis to inhibit the fungal adherence could be of a great interest for the biomedical field, opening new directions for the design of film-coated surfaces with antibiofilm properties.